Antibody Name:	Anti-HNRNPM Antibody
Antibody SKU:	CAB6937
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 491-730 of human HNRNPM (NP_005959.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HL-60, Mouse thymus, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 491-730 of human HNRNPM (NP_005959.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GFGL ERMA APID RVGQ TIER MGSG VERM GPAI ERMG LSME RMVP AGMG AGLE RMGP VMDR MATG LERM GANN LERM GLER MGAN SLER MGLE RMGA NSLE RMGP AMGP ALGA GIER MGLA MGGG GGAS FDRA IEME RGNF GGSF AGSF GGAG GHAP GVAR KACQ IFVR NLPF DFTW KMLK DKFN ECGH VLYA DIKM ENGK SKGC GVVK FESP EVAE RACR MMNG MKLS GREI DVRI DRNA
Gene ID:	4670
Uniprot:	P52272
Cellular Location:	Nucleus, nucleolus
Calculated MW:	73kDa/77kDa
Observed MW:	78kDa

Synonyms:

HNRNPM, CEAR, HNRNPM4, HNRPM, HNRPM4, HTGR1, NAGR1, hnRNP M, hnRNPM

Background:

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	hnRNP M: Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Receptor, misc.; RNA splicing; Spliceosome; Nucleolus Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: extracellular matrix; integral to plasma membrane; membrane; nuclear matrix; nucleoplasm; paraspeckles; spliceosome Molecular Function:protein binding; protein domain specific binding Biological Process: alternative nuclear mRNA splicing, via spliceosome; fibroblast growth factor receptor signaling pathway; gene expression; nuclear mRNA splicing, via spliceosome
NCBI Summary:	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
UniProt Code:	P52272
NCBI GenInfo Identifier:	55977747
NCBI Gene ID:	4670
NCBI Accession:	P52272.3
UniProt Secondary Accession:	P52272,Q15584, Q8WZ44, Q96H56, Q9BWL9, Q9Y492,
UniProt Related Accession:	P52272
Molecular Weight:	Da
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein M
NCBI Synonym Full Names:	heterogeneous nuclear ribonucleoprotein M
NCBI Official Symbol:	HNRNPM
NCBI Official Synonym Symbols:	CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein M
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein M
Protein Family:	Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:	HNRNPM
UniProt Entry Name:	HNRPM_HUMAN

	Western blot analysis of extracts of various cell lines, using HNRNPM antibody (CAB6937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse testis using hnRNPM antibody (CAB6937) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using hnRNPM antibody (CAB6937) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using hnRNPM antibody (CAB6937) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using hnRNPM antibody (CAB6937) at dilution of 1:100 (40x lens).