Antibody Name:	Anti-HNRNPH1 Antibody
Antibody SKU:	CAB5924
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human HNRNPH1

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat testis, Rat brain

Immunogen:	A synthetic peptide of human HNRNPH1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3187
Uniprot:	P31943
Cellular Location:	Nucleus, nucleoplasm
Calculated MW:	49kDa
Observed MW:	55kDa

Synonyms:

HNRNPH1, HNRPH, HNRPH1, hnRNPH

Background:

This gene encodes a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some may shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNA and is very similar to the family member HNRPF. This gene may be associated with hereditary lymphedema type I. Alternatively spliced transcript variants have been described

UniProt Protein Function:	hnRNP H1: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
UniProt Protein Details:	Protein type:RNA splicing; RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: nucleoplasm; membrane; nucleus Molecular Function:protein binding; RNA binding; nucleotide binding; poly(U) binding Biological Process: nuclear mRNA splicing, via spliceosome; RNA processing; regulation of RNA splicing; RNA splicing; gene expression
NCBI Summary:	This gene encodes a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some may shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNA and is very similar to the family member HNRPF. This gene may be associated with hereditary lymphedema type I. Alternatively spliced transcript variants have been described [provided by RefSeq, Mar 2012]
UniProt Code:	P31943
NCBI GenInfo Identifier:	1710632
NCBI Gene ID:	3187
NCBI Accession:	P31943.4
UniProt Secondary Accession:	P31943,Q6IBM4, B3KW86, D3DWQ2,
UniProt Related Accession:	P31943
Molecular Weight:	449
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein H
NCBI Synonym Full Names:	heterogeneous nuclear ribonucleoprotein H1 (H)
NCBI Official Symbol:	HNRNPH1
NCBI Official Synonym Symbols:	HNRPH; HNRPH1; hnRNPH
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein H
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein H
Protein Family:	Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:	HNRNPH1
UniProt Entry Name:	HNRH1_HUMAN

	Western blot analysis of extracts of various cell lines, using HNRNPH1 Rabbit pAb (CAB5924) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of 293T cells using HNRNPH1 antibody (CAB5924) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of 293T cells using HNRNPH1 Polyclonal Antibody (CAB5924) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.