Epigenetics & Nuclear Signaling Antibodies 3

Anti-HNRNPF Antibody (CAB5505)

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SKU:
CAB5505
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-HNRNPF Antibody
Antibody SKU:CAB5505
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPF (NP_001091678.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:22Rv1, Lovo, MCF7, BT-474, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPF (NP_001091678.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMLG PEGG EGFV VKLR GLPW SCSV EDVQ NFLS DCTI HDGA AGVH FIYT REGR QSGE AFVE LGSE DDVK MALK KDRE SMGH RYIE VFKS HRTE MDWV LKHS GPNS ADSA NDGF VRLR GLPF GCTK EEIV QFFS GLEI VPNG ITLP VDPE GKIT GEAF VQFA SQEL AEKA LGKH KERI GHRY IEVF KSSQ EEVR SYSD PPLK FMSV QRPG PYDR PGTA RRYI GIVK QAGL ERMR PGAY STGY GGYE EYSG LSDG YGFT TDLF GRDL SYCL SGMY DHRY GDSE
Gene ID:3185
Uniprot:P52597
Cellular Location:Nucleus, nucleoplasm
Calculated MW:45kDa
Observed MW:47kDa
Synonyms:HNRNPF, HNRPF, OK/SW-cl.23, mcs94-1
Background:This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
UniProt Protein Function:hnRNP F: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
UniProt Protein Details:

Protein type:Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function:protein binding; single-stranded RNA binding; RNA binding; nucleotide binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; regulation of RNA splicing; RNA splicing; gene expression

NCBI Summary:This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:P52597
NCBI GenInfo Identifier:1710628
NCBI Gene ID:3185
NCBI Accession:P52597.3
UniProt Secondary Accession:P52597,Q5T0N2, Q96AU2, B3KM84,
UniProt Related Accession:P52597
Molecular Weight:415
NCBI Full Name:Heterogeneous nuclear ribonucleoprotein F
NCBI Synonym Full Names:heterogeneous nuclear ribonucleoprotein F
NCBI Official Symbol:HNRNPF  
NCBI Official Synonym Symbols:HNRPF; mcs94-1; OK/SW-cl.23  
NCBI Protein Information:heterogeneous nuclear ribonucleoprotein F; HnRNP F protein; nucleolin-like protein mcs94-1
UniProt Protein Name:Heterogeneous nuclear ribonucleoprotein F
UniProt Synonym Protein Names:Nucleolin-like protein mcs94-1Heterogeneous nuclear ribonucleoprotein F, N-terminally processed
Protein Family:Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:HNRNPF  
UniProt Entry Name:HNRPF_HUMAN
Anti-HNRNPF Antibody (CAB5505)Western blot analysis of extracts of various cell lines, using HNRNPF antibody (CAB5505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-HNRNPF Antibody (CAB5505)Immunofluorescence analysis of U2OS cells using HNRNPF antibody (CAB5505). Blue: DAPI for nuclear staining.
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