Epigenetics & Nuclear Signaling Antibodies 3

Anti-HNF1A Antibody (CAB3092)

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SKU:
CAB3092
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-HNF1A Antibody
Antibody SKU:CAB3092
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human HNF1A
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, LO2
Immunogen:A synthetic peptide of human HNF1A
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6927
Uniprot:P20823
Cellular Location:Nucleus
Calculated MW:12kDa/27kDa/30kDa/43kDa/55-70kDa
Observed MW:81KDa
Synonyms:HNF1A, HNF-1A, HNF1, IDDM20, LFB1, MODY3, TCF-1, TCF1
Background:The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:HNF1A: Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'- GTTAATNATTAAC-3'. Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF). Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3); also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20). IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the HNF1 homeobox family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus

Molecular Function:protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; insulin secretion; glucose import; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis

Disease: Diabetes Mellitus, Insulin-dependent, 20; Diabetes Mellitus, Insulin-dependent; Hepatic Adenomas, Familial; Maturity-onset Diabetes Of The Young, Type 3; Renal Cell Carcinoma, Nonpapillary; Diabetes Mellitus, Noninsulin-dependent

NCBI Summary:The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
UniProt Code:P20823
NCBI GenInfo Identifier:51338763
NCBI Gene ID:6927
NCBI Accession:P20823.2
UniProt Secondary Accession:P20823,Q2M3H2, Q99861, A5Z2R8, E0YMJ5, E0YMK0, E0YMK1 E2I9R4, E2I9R5, F5H5U3,
UniProt Related Accession:P20823
Molecular Weight:12,491 Da
NCBI Full Name:Hepatocyte nuclear factor 1-alpha
NCBI Synonym Full Names:HNF1 homeobox A
NCBI Official Symbol:HNF1A  
NCBI Official Synonym Symbols:HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20  
NCBI Protein Information:hepatocyte nuclear factor 1-alpha
UniProt Protein Name:Hepatocyte nuclear factor 1-alpha
UniProt Synonym Protein Names:Liver-specific transcription factor LF-B1; LFB1; Transcription factor 1; TCF-1
Protein Family:Hepatocyte nuclear factor
UniProt Gene Name:HNF1A  
UniProt Entry Name:HNF1A_HUMAN
Anti-HNF1A Antibody (CAB3092)Western blot analysis of extracts of various cell lines, using HNF1A antibody (CAB3092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
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