Epigenetics & Nuclear Signaling Antibodies 1

Anti-HMGA1 Antibody (CAB12693)

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SKU:
CAB12693
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-HMGA1 Antibody
Antibody SKU:CAB12693
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1).
Application:WB IHC
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Rat kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSES SSKS SQPL ASKQ EKDG TEKR GRGR PRKQ PPVS PGTA LVGS QKEP SEVP TPKR PRGR PKGS KNKG AAKT RKTT TTPG RKPR GRPK KLEK EEEE GISQ
Gene ID:3159
Uniprot:P17096
Cellular Location:Chromosome, Nucleus
Calculated MW:10kDa/11kDa/19kDa
Observed MW:16kDa
Synonyms:HMGA1, HMG-R, HMGA1A, HMGIY
Background:This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes.
UniProt Protein Function:HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; transcription factor complex; focal adhesion; cytosol; nucleus

Molecular Function:retinoid X receptor binding; peroxisome proliferator activated receptor binding; protein binding; enzyme binding; DNA-(apurinic or apyrimidinic site) lyase activity; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; AT DNA binding; retinoic acid receptor binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding

Biological Process: DNA unwinding during replication; viral reproduction; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; response to virus; DNA catabolic process, endonucleolytic; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; nucleosome disassembly; negative regulation of chromatin silencing; base-excision repair; protein complex assembly; negative regulation of transcription, DNA-dependent

NCBI Summary:This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P17096
NCBI GenInfo Identifier:123377
NCBI Gene ID:3159
NCBI Accession:P17096.3
UniProt Secondary Accession:P17096,P10910, Q5T6U9, Q9UKB0,
UniProt Related Accession:P17096
Molecular Weight:11,676 Da
NCBI Full Name:High mobility group protein HMG-I/HMG-Y
NCBI Synonym Full Names:high mobility group AT-hook 1
NCBI Official Symbol:HMGA1  
NCBI Official Synonym Symbols:HMG-R; HMGIY; HMGA1A  
NCBI Protein Information:high mobility group protein HMG-I/HMG-Y; HMG-I(Y); high mobility group protein R; high mobility group protein A1; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y; high-mobility group (nonhistone chromosomal) protein isoforms I and Y
UniProt Protein Name:High mobility group protein HMG-I/HMG-Y
UniProt Synonym Protein Names:High mobility group AT-hook protein 1; High mobility group protein A1; High mobility group protein R
Protein Family:High mobility group protein
UniProt Gene Name:HMGA1  
UniProt Entry Name:HMGA1_HUMAN
Anti-HMGA1 Antibody (CAB12693)Western blot analysis of extracts of rat kidney, using HMGA1 antibody (CAB12693) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-HMGA1 Antibody (CAB12693)Immunohistochemistry of paraffin-embedded mouse brain using HMGA1 antibody (CAB12693) at dilution of 1:100 (40x lens).
Anti-HMGA1 Antibody (CAB12693)Immunohistochemistry of paraffin-embedded mouse kidney using HMGA1 antibody (CAB12693) at dilution of 1:100 (40x lens).
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