Epigenetics & Nuclear Signaling Antibodies 4
Anti-HMG20A Antibody (CAB7286)
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- SKU:
- CAB7286
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HMG20A Antibody |
| Antibody SKU: | CAB7286 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-347 of human HMG20A (NP_060670.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, 22Rv1, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-347 of human HMG20A (NP_060670.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MENL MTSS TLPP LFAD EDGS KESN DLAT TGLN HPEV PYSS GATS STNN PEFV EDLS QGQL LQSE SSNA AEGN EQRH EDEQ RSKR GGWS KGRK RKKP LRDS NAPK SPLT GYVR FMNE RREQ LRAK RPEV PFPE ITRM LGNE WSKL PPEE KQRY LDEA DRDK ERYM KELE QYQK TEAY KVFS RKTQ DRQK GKSH RQDA ARQA THDH EKET EVKE RSVF DIPI FTEE FLNH SKAR EAEL RQLR KSNM EFEE RNAA LQKH VESM RTAV EKLE VDVI QERS RNTV LQQH LETL RQVL TSSF ASMP LPGS GETP TVDT IDSY MNRL HSII LANP QDNE NFIA TVRE VVNR LDR |
| Gene ID: | 10363 |
| Uniprot: | Q9NP66 |
| Cellular Location: | Nucleus |
| Calculated MW: | 8kDa/40kDa |
| Observed MW: | 41kDa |
| Synonyms: | HMG20A, HMGX1, HMGXB1 |
| Background: |
| UniProt Protein Function: | HMG20A: Plays a role in neuronal differentiation as chromatin- associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 15q24 Cellular Component: nucleus Molecular Function:protein binding; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | Q9NP66 |
| NCBI GenInfo Identifier: | 74734297 |
| NCBI Gene ID: | 10363 |
| NCBI Accession: | Q9NP66.1 |
| UniProt Secondary Accession: | Q9NP66,Q53G31, Q9NSF6, A6NHY3, D3DW78, |
| UniProt Related Accession: | Q9NP66 |
| Molecular Weight: | 347 |
| NCBI Full Name: | High mobility group protein 20A |
| NCBI Synonym Full Names: | high mobility group 20A |
| NCBI Official Symbol: | HMG20A |
| NCBI Official Synonym Symbols: | HMGX1; HMGXB1 |
| NCBI Protein Information: | high mobility group protein 20A; high-mobility group 20A; HMG box domain containing 1; HMG box-containing protein 20A; HMG domain-containing protein 1; HMG domain-containing protein HMGX1 |
| UniProt Protein Name: | High mobility group protein 20A |
| UniProt Synonym Protein Names: | HMG box-containing protein 20A; HMG domain-containing protein 1; HMG domain-containing protein HMGX1 |
| Protein Family: | High mobility group protein |
| UniProt Gene Name: | HMG20A |
| UniProt Entry Name: | HM20A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HMG20A antibody (CAB7286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using HMG20A antibody (CAB7286) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using HMG20A antibody (CAB7286). |
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