Name: Anti-Histone H3.3 Antibody (CAB4835)
Brand: Cell Biology Antibodies 17
SKU: CAB4835
Availability: OutOfStock

Product Name:	Histone H3.3 Rabbit mAb
Product Code:	CAB4835
Size:	20uL, 50uL, 100uL
Synonyms:	H3.3A, H3F3
Applications:	WB, IHC
Reactivity:	Human, Mouse, Rat, Other (Wide Range)
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human Histone H3.3

Applications:	WB, IHC
Recommended Dilutions:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat, Other (Wide Range)
Positive Samples:	HeLa, 294T, Mouse lungRat lung

Immunogen:	A synthesized peptide derived from human Histone H3.3
Purification Method:	Affinity purification
Storage:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3020
Uniprot:	P84243
Cellular Location:	Chromosome, Nucleus
Calculated MW:	15kDa
Observed MW:	17KDa

UniProt Protein Function:	H3F3A: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Belongs to the histone H3 family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q42.12 Cellular Component: extracellular region; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:histone binding; nucleosomal DNA binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of cell growth; positive regulation of gene expression, epigenetic; RNA-mediated gene silencing; telomere organization and biogenesis
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17. [provided by RefSeq, Oct 2015]
UniProt Code:	P84243
NCBI GenInfo Identifier:	55977062
NCBI Gene ID:	3021
NCBI Accession:	P84243.2
UniProt Secondary Accession:	P84243,P06351, P33155, Q5VV55, Q5VV56, Q66I33, Q9V3W4
UniProt Related Accession:	P84243
Molecular Weight:	15,328 Da
NCBI Full Name:	Histone H3.3
NCBI Synonym Full Names:	H3 histone, family 3B
NCBI Official Symbol:	H3F3B
NCBI Official Synonym Symbols:	H3.3B
NCBI Protein Information:	histone H3.3
UniProt Protein Name:	Histone H3.3
UniProt Gene Name:	H3F3A
UniProt Entry Name:	H33_HUMAN

	Western blot analysis of extracts of Rat lung, using Histone H3. 3 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using Histone H3. 3 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat kidney using Histone H3. 3 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human esophageal using Histone H3. 3 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse liver using Histone H3. 3 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol.
	Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H3. 3 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.