Anti-HINT1 Antibody (CAB20894)

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SKU:
CAB20894
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-HINT1 Antibody (CAB20894)
Antibody SKU:CAB20894
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human HINT1.
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Jurkat, K-562, Mouse brain, Mouse kidney, Mouse pancreas, Rat liver, Rat testis
Immunogen:Recombinant protein of Human HINT1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Cytoplasm, Nucleus
Calculated MW:13kDa
Observed MW:14KDa
Synonyms:HINT1, HINT, NMAN, PKCI-1, PRKCNH1
Background:This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed.
Immunofluorescence analysis of NIH/3T3 cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC-12 cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Jurkat cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of Jurkat cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF7 cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of MCF7 cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HepG2 cells using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human colon carcinoma using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human colon carcinoma using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human breast cancer using HINT1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using HINT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Western blot analysis of extracts of various cell lines, using HINT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using HINT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Western blot analysis of extracts of various cell lines, using HINT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
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