Cell Biology Antibodies 5
Anti-HIGD1A Antibody (CAB14582)
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- SKU:
- CAB14582
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HIGD1A Antibody |
| Antibody SKU: | CAB14582 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-93 of human HIGD1A (NP_054775.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | HeLa, HepG2, 293T, K-562 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-93 of human HIGD1A (NP_054775.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSTD TGVS LPSY EEDQ GSKL IRKA KEAP FVPV GIAG FAAI VAYG LYKL KSRG NTKM SIHL IHMR VAAQ GFVV GAMT VGMG YSMY REFW AKPK P |
| Gene ID: | 25994 |
| Uniprot: | Q9Y241 |
| Cellular Location: | Mitochondrion inner membrane, Mitochondrion membrane, Multi-pass membrane protein |
| Calculated MW: | 10kDa/11kDa |
| Observed MW: | 14kDa |
| Synonyms: | HIGD1A, HIG1, RCF1a |
| Background: |
| UniProt Protein Function: | HIGD1A: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p22.1 Cellular Component: protein complex; mitochondrial inner membrane; integral to membrane Biological Process: cellular response to glucose starvation; negative regulation of apoptosis |
| UniProt Code: | Q9Y241 |
| NCBI GenInfo Identifier: | 153085461 |
| NCBI Gene ID: | 25994 |
| NCBI Accession: | NP_001093138 |
| UniProt Secondary Accession: | Q9Y241,Q9UFZ2, |
| UniProt Related Accession: | Q9Y241 |
| Molecular Weight: | 11,770 Da |
| NCBI Full Name: | HIG1 domain family member 1A, mitochondrial isoform a |
| NCBI Synonym Full Names: | HIG1 hypoxia inducible domain family, member 1A |
| NCBI Official Symbol: | HIGD1A |
| NCBI Official Synonym Symbols: | HIG1; RCF1a |
| NCBI Protein Information: | HIG1 domain family member 1A, mitochondrial |
| UniProt Protein Name: | HIG1 domain family member 1A, mitochondrial |
| UniProt Synonym Protein Names: | Hypoxia-inducible gene 1 protein; RCF1 homolog A; RCF1a |
| Protein Family: | HIG1 domain family |
| UniProt Gene Name: | HIGD1A |
| UniProt Entry Name: | HIG1A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HIGD1A antibody (CAB14582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using HIGD1A antibody (CAB14582) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using HIGD1A Polyclonal Antibody (CAB14582) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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