Cell Biology Antibodies 15

Anti-HIF3A Antibody (CAB20020)

(No reviews yet) Write a Review
SKU:
CAB20020
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-HIF3A Antibody
Antibody SKU:CAB20020
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human HIF3A.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HUVEC, mouse heart, rat brain, rat liver
Immunogen:Recombinant protein of human HIF3A.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:64344
Uniprot:Q9Y2N7
Cellular Location:Cytoplasm, Nucleus
Calculated MW:
Observed MW:64kDa
Synonyms:HIF-3A, HIF3-alpha-1, IPAS, MOP7, PASD7, bHLHe17
Background:The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
UniProt Protein Function:HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; cytosol

Molecular Function:protein dimerization activity; signal transducer activity; DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; signal transduction

NCBI Summary:The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
UniProt Code:Q9Y2N7
NCBI GenInfo Identifier:145558932
NCBI Gene ID:64344
NCBI Accession:Q9Y2N7.2
UniProt Secondary Accession:Q9Y2N7,Q58A43, Q66K72, Q8WXA1, Q96K34, Q9H7Z9, Q9HAI2 B0M185, B4DNA2,
UniProt Related Accession:Q9Y2N7
Molecular Weight:64,359 Da
NCBI Full Name:Hypoxia-inducible factor 3-alpha
NCBI Synonym Full Names:hypoxia inducible factor 3, alpha subunit
NCBI Official Symbol:HIF3A  
NCBI Official Synonym Symbols:IPAS; MOP7; PASD7; HIF-3A; bHLHe17  
NCBI Protein Information:hypoxia-inducible factor 3-alpha; HIF3-alpha-1; member of PAS protein 7; inhibitory PAS domain protein; PAS domain-containing protein 7; basic-helix-loop-helix-PAS protein MOP7; class E basic helix-loop-helix protein 17
UniProt Protein Name:Hypoxia-inducible factor 3-alpha
UniProt Synonym Protein Names:Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-containing protein 7
Protein Family:Hypoxia-inducible factor
UniProt Gene Name:HIF3A  
UniProt Entry Name:HIF3A_HUMAN
Anti-HIF3A Antibody (CAB20020)Western blot - HIF3A Rabbit pAb (CAB20020)
View AllClose

0 Reviews

View AllClose

Related Products