KO Validated Antibodies 2

Anti-HIF1AN/FIH1 Antibody [KO Validated] (CAB3701)

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SKU:
CAB3701

Description

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Antibody Name:Anti-HIF1AN/FIH1 Antibody [KO Validated]
Antibody SKU:CAB3701
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human HIF1AN/FIH1
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, 293T
Immunogen:A synthesized peptide derived from human HIF1AN/FIH1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:55662
Uniprot:Q9NWT6
Cellular Location:
Calculated MW:40kDa
Observed MW:40KDa
Synonyms:FIH1
Background:
UniProt Protein Function:HIF1AN: Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A. Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4.
UniProt Protein Details:

Protein type:EC 1.14.11.30; EC 1.14.11.n4; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function:NF-kappaB binding; protein binding; protein homodimerization activity; oxygen sensor activity; zinc ion binding; carboxylic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; Notch binding; cofactor binding

Biological Process: peptidyl-aspartic acid hydroxylation; transcription, DNA-dependent; peptidyl-asparagine hydroxylation; negative regulation of Notch signaling pathway; positive regulation of myoblast differentiation

UniProt Code:Q9NWT6
NCBI GenInfo Identifier:32129605
NCBI Gene ID:55662
NCBI Accession:Q9NWT6.2
UniProt Secondary Accession:Q9NWT6,Q5W147, Q969Q7, Q9NPV5, D3DR69,
UniProt Related Accession:Q9NWT6
Molecular Weight:40,285 Da
NCBI Full Name:Hypoxia-inducible factor 1-alpha inhibitor
NCBI Synonym Full Names:hypoxia inducible factor 1, alpha subunit inhibitor
NCBI Official Symbol:HIF1AN  
NCBI Official Synonym Symbols:FIH1  
NCBI Protein Information:hypoxia-inducible factor 1-alpha inhibitor; FIH-1; factor inhibiting HIF-1; factor inhibiting HIF1; hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
UniProt Protein Name:Hypoxia-inducible factor 1-alpha inhibitor
UniProt Synonym Protein Names:Factor inhibiting HIF-1; FIH-1; Hypoxia-inducible factor asparagine hydroxylase
Protein Family:Hypoxia-inducible factor 1-alpha inhibitor
UniProt Gene Name:HIF1AN  
UniProt Entry Name:HIF1N_HUMAN
Anti-HIF1AN/FIH1 Antibody [KO Validated] (CAB3701)Western blot analysis of extracts from normal (control) and HIF1AN/FIH1 knockout (KO) 293T cells, using HIF1AN/FIH1 antibody (CAB3701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Anti-HIF1AN/FIH1 Antibody [KO Validated] (CAB3701)Western blot analysis of extracts of U-87MG cells, using HIF1AN/FIH1 Rabbit mAb (CAB3701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
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Additional Information

Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
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