Signal Transduction Antibodies 2

Anti-HEY2 Antibody (CAB15143)

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SKU:
CAB15143
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-HEY2 Antibody
Antibody SKU:CAB15143
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HEY2 (NP_036391.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, K562, MCF7, mouse brain, rat heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HEY2 (NP_036391.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKRP CEET TSES DMDE TIDV GSEN NYSG QSTS SVIR LNSP TTTS QIMA RKKR RGII EKRR RDRI NNSL SELR RLVP TAFE KQGS AKLE KAEI LQMT VDHL
Gene ID:23493
Uniprot:Q9UBP5
Cellular Location:Nucleus
Calculated MW:35kDa
Observed MW:36kDa
Synonyms:HEY2, CHF1, GRIDLOCK, GRL, HERP1, HESR2, HRT2, bHLHb32
Background:This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found, but their biological validity has not been determined.
UniProt Protein Function:HEY2: Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'- CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Belongs to the HEY family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; nucleoplasm; nucleus; Sin3 complex; transcriptional repressor complex

Molecular Function:histone deacetylase binding; protein binding; protein dimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: anterior/posterior axis specification; cardiac muscle hypertrophy; cell fate commitment; mesenchymal cell development; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; Notch signaling pathway; positive regulation of cardiac muscle cell proliferation; positive regulation of heart rate; positive regulation of transcription from RNA polymerase II promoter; protein-DNA complex assembly; regulation of auditory receptor cell differentiation; smooth muscle cell differentiation; transcription, DNA-dependent; vasculogenesis; ventricular cardiac muscle cell development

NCBI Summary:This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UBP5
NCBI GenInfo Identifier:74762767
NCBI Gene ID:23493
NCBI Accession:Q9UBP5.1
UniProt Secondary Accession:Q9UBP5,Q9QUS4,
UniProt Related Accession:Q9UBP5
Molecular Weight:35,808 Da
NCBI Full Name:Hairy/enhancer-of-split related with YRPW motif protein 2
NCBI Synonym Full Names:hes related family bHLH transcription factor with YRPW motif 2
NCBI Official Symbol:HEY2  
NCBI Official Synonym Symbols:GRL; CHF1; HRT2; HERP1; HESR2; bHLHb32; GRIDLOCK  
NCBI Protein Information:hairy/enhancer-of-split related with YRPW motif protein 2
UniProt Protein Name:Hairy/enhancer-of-split related with YRPW motif protein 2
UniProt Synonym Protein Names:Cardiovascular helix-loop-helix factor 1; hCHF1; Class B basic helix-loop-helix protein 32; bHLHb32; HES-related repressor protein 2; Hairy and enhancer of split-related protein 2; HESR-2; Hairy-related transcription factor 2; HRT-2; hHRT2; Protein gridlock homolog
Protein Family:Hairy/enhancer-of-split related with YRPW motif protein
UniProt Gene Name:HEY2  
UniProt Entry Name:HEY2_HUMAN
Anti-HEY2 Antibody (CAB15143)Western blot analysis of extracts of various cell lines, using HEY2 antibody (CAB15143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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