Name: Anti-HES6 Antibody (CAB16547)
Brand: Developmental Biology
SKU: CAB16547
Availability: OutOfStock

Antibody Name:	Anti-HES6 Antibody
Antibody SKU:	CAB16547
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human HES6 (NP_061115.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human HES6 (NP_061115.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPP AAPG RDRV GRED EDGW ETRG DRKA RKPL VEKK RRAR INES LQEL RLLL AGAE VQAK LENA EVLE LTVR RVQG VLRG RARE REQL QAEA SERF AAGY IQCM HEVH TFVS TCQA IDAT VAAE LLNH LLES MPLR EGSS FQDL LGDA LAGP PRAP GRSG WPAG GAPG SPIP SPPG PGDD LCSD LEEA PEAE LSQA PAEG PDLV PAAL GSLT TAQI ARSV WRPW
Gene ID:	55502
Uniprot:	Q96HZ4
Cellular Location:	Nucleus
Calculated MW:	12kDa/23kDa/24kDa
Observed MW:	26kDa

Synonyms:

HES6, C-HAIRY1, HES-6, bHLHb41, bHLHc23

Background:

This gene encodes a member of a subfamily of basic helix-loop-helix transcription repressors that have homology to the Drosophila enhancer of split genes. Members of this gene family regulate cell differentiation in numerous cell types. The protein encoded by this gene functions as a cofactor, interacting with other transcription factors through a tetrapeptide domain in its C-terminus. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	HES6: Does not bind DNA itself but suppresses both HES1- mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: transcription factor complex Molecular Function:transcription cofactor activity; transcription factor activity Biological Process: regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of a subfamily of basic helix-loop-helix transcription repressors that have homology to the Drosophila enhancer of split genes. Members of this gene family regulate cell differentiation in numerous cell types. The protein encoded by this gene functions as a cofactor, interacting with other transcription factors through a tetrapeptide domain in its C-terminus. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Dec 2008]
UniProt Code:	Q96HZ4
NCBI GenInfo Identifier:	50400609
NCBI Gene ID:	55502
NCBI Accession:	Q96HZ4.1
UniProt Secondary Accession:	Q96HZ4,Q53SN9, Q8N2J2, Q96T93, Q9P2S3, A8KAP6, B8ZZA9
UniProt Related Accession:	Q96HZ4
Molecular Weight:	12,240 Da
NCBI Full Name:	Transcription cofactor HES-6
NCBI Synonym Full Names:	hes family bHLH transcription factor 6
NCBI Official Symbol:	HES6
NCBI Official Synonym Symbols:	HES-6; bHLHb41; bHLHc23; C-HAIRY1
NCBI Protein Information:	transcription cofactor HES-6
UniProt Protein Name:	Transcription cofactor HES-6
UniProt Synonym Protein Names:	C-HAIRY1; Class B basic helix-loop-helix protein 41; bHLHb41; Hairy and enhancer of split 6
Protein Family:	Transcription cofactor
UniProt Gene Name:	HES6
UniProt Entry Name:	HES6_HUMAN

Western blot analysis of extracts of 293T cells, using HES6 antibody (CAB16547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

HES6 Antibody (PACO00904)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)