Signal Transduction Antibodies 3

Anti-HERC3 Antibody (CAB8567)

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SKU:
CAB8567
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-HERC3 Antibody
Antibody SKU:CAB8567
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HERC3 (NP_055421.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:SH-SY5Y, U-87MG, A-431, Mouse lung, Mouse spinal cord
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HERC3 (NP_055421.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLCW GYWS LGQP GIST NLQG IVAE PQVC GFIS DRSV KEVA CGGN HSVF LLED GEVY TCGL NTKG QLGH EREG NKPE QIGA LADQ HIIH VACG ESHS LALS DRGQ LFSW GAGS DGQL GLMT TEDS VAVP RLIQ KLNQ QTIL QVSC GNWH CLAL AADG QFFT WGKN SHGQ LGLG KEFP SQAS PQRV RSLE GIPL AQVA AGGA
Gene ID:8916
Uniprot:Q15034
Cellular Location:Cytoplasm, Cytoplasmic vesicle
Calculated MW:39kDa/117kDa
Observed MW:117kDa
Synonyms:HERC3
Background:This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:HERC3: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
UniProt Protein Details:

Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: cytoplasm; nucleus

Molecular Function:ubiquitin-protein ligase activity

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process

NCBI Summary:This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]
UniProt Code:Q15034
NCBI GenInfo Identifier:2495699
NCBI Gene ID:8916
NCBI Accession:Q15034.1
UniProt Secondary Accession:Q15034,Q8IXX3, A8K1S5,
UniProt Related Accession:Q15034
Molecular Weight:39,051 Da
NCBI Full Name:Probable E3 ubiquitin-protein ligase HERC3
NCBI Synonym Full Names:HECT and RLD domain containing E3 ubiquitin protein ligase 3
NCBI Official Symbol:HERC3  
NCBI Protein Information:probable E3 ubiquitin-protein ligase HERC3
UniProt Protein Name:Probable E3 ubiquitin-protein ligase HERC3
UniProt Synonym Protein Names:HECT domain and RCC1-like domain-containing protein 3
Protein Family:Probable E3 ubiquitin-protein ligase
UniProt Gene Name:HERC3  
UniProt Entry Name:HERC3_HUMAN
Anti-HERC3 Polyclonal Antibody (CAB8567)Western blot analysis of extracts of various cell lines, using HERC3 antibody (CAB8567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-HERC3 Polyclonal Antibody (CAB8567)Immunohistochemistry of paraffin-embedded human prostate using HERC3 antibody (CAB8567) at dilution of 1:100 (40x lens).
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