Epigenetics & Nuclear Signaling Antibodies 3
Anti-HDGF Antibody (CAB5347)
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- SKU:
- CAB5347
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HDGF Antibody |
| Antibody SKU: | CAB5347 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, HeLa, A-549, 293T, U-251MG, MCF7, BT-474 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSRS NRQK EYKC GDLV FAKM KGYP HWPA RIDE MPEA AVKS TANK YQVF FFGT HETA FLGP KDLF PYEE SKEK FGKP NKRK GFSE GLWE IENN PTVK ASGY QSSQ KKSC VEEP EPEP EAAE GDGD KKGN AEGS SDEE GKLV IDEP AKEK NEKG ALKR RAGD LLED SPKR PKEA ENPE GEEK EAAT LEVE RPLP MEVE KNST PSEP GSGR GPPQ EEEE EEDE EEEA TKED AEAP GIRD HESL |
| Gene ID: | 3068 |
| Uniprot: | P51858 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 25kDa/26kDa/28kDa |
| Observed MW: | 38kDa |
| Synonyms: | HDGF, HMG1L2 |
| Background: | This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. |
| UniProt Protein Function: | Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. |
| NCBI Summary: | This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016] |
| UniProt Code: | P51858 |
| NCBI GenInfo Identifier: | 1708157 |
| NCBI Gene ID: | 3068 |
| NCBI Accession: | P51858.1 |
| UniProt Secondary Accession: | P51858,Q5SZ07, Q5SZ08, Q5SZ09, B3KU21, D3DVC9, |
| UniProt Related Accession: | P51858 |
| Molecular Weight: | 28,264 Da |
| NCBI Full Name: | Hepatoma-derived growth factor |
| NCBI Synonym Full Names: | hepatoma-derived growth factor |
| NCBI Official Symbol: | HDGF |
| NCBI Official Synonym Symbols: | HMG1L2 |
| NCBI Protein Information: | hepatoma-derived growth factor |
| UniProt Protein Name: | Hepatoma-derived growth factor |
| UniProt Synonym Protein Names: | High mobility group protein 1-like 2; HMG-1L2 |
| Protein Family: | Hepatoma-derived growth factor |
| UniProt Gene Name: | HDGF |
| UniProt Entry Name: | HDGF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HDGF antibody (CAB5347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody (CAB5347) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using HDGF Antibody (CAB5347) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using HDGF Antibody (CAB5347) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using HDGF antibody (CAB5347) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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