Signal Transduction Antibodies 3
Anti-HCN1 Antibody (CAB17279)
- SKU:
- CAB17279
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
Antibody Name: | Anti-HCN1 Antibody (CAB17279) |
Antibody SKU: | CAB17279 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 800-890 of human HCN1 (NP_066550.2). |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | SH-SY5Y, Neuro-2a, Mouse brain, Rat brain |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 800-890 of human HCN1 (NP_066550.2). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | LISR PHPT VGES LASI PQPV TAVP GTGL QAGG RSTV PQRV TLFR QMSS GAIP PNRG VPPA PPPP AAAL PRES SSVL NTDP DAEK PRFA SNL |
Cellular Location: | Cell membrane, Multi-pass membrane protein |
Calculated MW: | 98kDa |
Observed MW: | 99KDa |
Synonyms: | HCN1, BCNG-1, BCNG1, EIEE24, HAC-2 |
Background: | The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. |
Western blot analysis of extracts of various cell lines, using HCN1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |