Name: Anti-HCN1 Antibody (CAB16198)
Brand: Signal Transduction Antibodies 2
SKU: CAB16198
Availability: OutOfStock

Antibody Name:	Anti-HCN1 Antibody
Antibody SKU:	CAB16198
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human HCN1

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	A synthetic peptide of human HCN1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	348980
Uniprot:	O60741
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	98kDa
Observed MW:	113kDa

Synonyms:	HCN1, BCNG-1, BCNG1, EIEE24, HAC-2
Background:	The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes.

UniProt Protein Function:	HCN1: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. Belongs to the potassium channel HCN family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 5p12 Cellular Component: axon; integral to plasma membrane; dendrite; integral to membrane; plasma membrane Molecular Function:identical protein binding; voltage-gated potassium channel activity; intracellular cAMP activated cation channel activity; potassium channel activity; voltage-gated sodium channel activity; cAMP binding Biological Process: synaptic transmission; regulation of membrane potential; apical protein localization; retinal cone cell development; potassium ion transport Disease: Epileptic Encephalopathy, Early Infantile, 24
NCBI Summary:	The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. [provided by RefSeq, Oct 2011]
UniProt Code:	O60741
NCBI GenInfo Identifier:	229462966
NCBI Gene ID:	348980
NCBI Accession:	O60741.3
UniProt Related Accession:	O60741
Molecular Weight:	890
NCBI Full Name:	Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
NCBI Synonym Full Names:	hyperpolarization activated cyclic nucleotide-gated potassium channel 1
NCBI Official Symbol:	HCN1
NCBI Official Synonym Symbols:	BCNG1; HAC-2; BCNG-1; EIEE24
NCBI Protein Information:	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1; brain cyclic nucleotide gated channel 1; brain cyclic nucleotide-gated channel 1
UniProt Protein Name:	Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
UniProt Synonym Protein Names:	Brain cyclic nucleotide-gated channel 1; BCNG-1
Protein Family:	Anaphase-promoting complex
UniProt Gene Name:	HCN1
UniProt Entry Name:	HCN1_HUMAN

	Western blot analysis of extracts of various cell lines, using HCN1 antibody (CAB16198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of NIH/3T3 cells using HCN1 Rabbit pAb (CAB16198) at dilution of 1:100. Blue: DAPI for nuclear staining.