Antibody Name:	Anti-HAUSP / USP7 Antibody
Antibody SKU:	CAB13564
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 206-1102 of human HAUSP / USP7 (NP_003461.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 206-1102 of human HAUSP / USP7 (NP_003461.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DSKK HTGY VGLK NQGA TCYM NSLL QTLF FTNQ LRKA VYMM PTEG DDSS KSVP LALQ RVFY ELQH SDKP VGTK KLTK SFGW ETLD SFMQ HDVQ ELCR VLLD NVEN KMKG TCVE GTIP KLFR GKMV SYIQ CKEV DYRS DRRE DYYD IQLS IKGK KNIF ESFV DYVA VEQL DGDN KYDA GEHG LQEA EKGV KFLT LPPV LHLQ LMRF MYDP QTDQ NIKI NDRF EFPE QLPL DEFL QKTD PKDP ANYI LHAV LVHS GDNH GGHY VVYL NPKG DGKW CKFD DDVV SRCT KEEA IEHN YGGH DDDL SVRH CTNA YMLV YIRE SKLS EVLQ AVTD HDIP QQLV ERLQ EEKR IEAQ KRKE RQEA HLYM QVQI VAED QFCG HQGN DMYD EEKV KYTV FKVL KNSS LAEF VQSL SQTM GFPQ DQIR LWPM QARS NGTK RPAM LDNE ADGN KTMI ELSD NENP WTIF LETV DPEL AASG ATLP KFDK DHDV MLFL KMYD PKTR SLNY CGHI YTPI SCKI RDLL PVMC DRAG FIQD TSLI LYEE VKPN LTER IQDY DVSL DKAL DELM DGDI IVFQ KDDP ENDN SELP TAKE YFRD LYHR VDVI FCDK TIPN DPGF VVTL SNRM NYFQ VAKT VAQR LNTD PMLL QFFK SQGY RDGP GNPL RHNY EGTL RDLL QFFK PRQP KKLY YQQL KMKI TDFE NRRS FKCI WLNS QFRE EEIT LYPD KHGC VRDL LEEC KKAV ELGE KASG KLRL LEIV SYKI IGVH QEDE LLEC LSPA TSRT FRIE EIPL DQVD IDKE NEML VTVA HFHK EVFG TFGI PFLL RIHQ GEHF REVM KRIQ SLLD IQEK EFEK FKFA IVMM GRHQ YINE DEYE VNLK DFEP QPGN MSHP RPWL GLDH FNKA PKRS RYTY LEKA IKIH N
Gene ID:	7874
Uniprot:	Q93009
Cellular Location:	Cytoplasm, Nucleus, PML body
Calculated MW:	126kDa/128kDa
Observed MW:	138kDa

Synonyms:

USP7, HAUSP, TEF1

Background:

The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.

UniProt Protein Function:	USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.
UniProt Protein Details:	Protein type:Ubiquitin-specific protease; Protease; EC 3.4.19.12; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nuclear body; PML body; cytosol; nucleus Molecular Function:protein C-terminus binding; protein binding; p53 binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding; ubiquitin-specific protease activity; transcription factor binding Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; maintenance of DNA methylation; viral reproduction; regulation of transcription factor activity; inhibition of NF-kappaB transcription factor; transcription-coupled nucleotide-excision repair; multicellular organismal development; histone deubiquitination
UniProt Code:	Q93009
NCBI GenInfo Identifier:	212276477
NCBI Gene ID:	7874
NCBI Accession:	Q93009.2
UniProt Secondary Accession:	Q93009,A6NMY8, B7Z815, H0Y3G8,
UniProt Related Accession:	Q93009
Molecular Weight:	1102
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 7
NCBI Synonym Full Names:	ubiquitin specific peptidase 7 (herpes virus-associated)
NCBI Official Symbol:	USP7
NCBI Official Synonym Symbols:	TEF1; HAUSP
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 7; ubiquitin thioesterase 7; deubiquitinating enzyme 7; ubiquitin-specific-processing protease 7; herpesvirus-associated ubiquitin-specific protease; Herpes virus-associated ubiquitin-specific protease; ubiquitin spec
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 7
UniProt Synonym Protein Names:	Deubiquitinating enzyme 7; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7
UniProt Gene Name:	USP7
UniProt Entry Name:	UBP7_HUMAN

	Western blot analysis of extracts of various cell lines, using HAUSP / USP7 antibody (CAB13564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human kidney cancer using HAUSP / USP7 antibody (CAB13564) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using HAUSP / USP7 antibody (CAB13564) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using HAUSP / USP7 antibody (CAB13564).