Name: Anti-GYPB Antibody (CAB7682)
Brand: Cell Biology Antibodies 11
SKU: CAB7682
Availability: OutOfStock

Antibody Name:	Anti-GYPB Antibody
Antibody SKU:	CAB7682
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-91 of human GYPB (NP_002091.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Human serum

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-91 of human GYPB (NP_002091.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MYGK IIFV LLLS EIVS ISAL STTE VAMH TSTS SSVT KSYI SSQT NGET GQLV HRFT VPAP VVII LIIL CVMA GIIG TILL ISYT IRRL IKA
Gene ID:	2994
Uniprot:	P06028
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	6kDa/9kDa
Observed MW:	25kDa

Synonyms:

GYPB, CD235b, GPB, GYP, MNS, PAS-3, SS

Background:

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPB gene consists of 5 exons and has 97% sequence homology with GYPA from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	GYPB: This protein is a minor sialoglycoprotein in erythrocyte membranes. Belongs to the glycophorin-A family.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 4q31.21 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:protein binding Disease: Malaria, Susceptibility To
NCBI Summary:	Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPB gene consists of 5 exons and has 97% sequence homology with GYPA from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
UniProt Code:	P06028
NCBI GenInfo Identifier:	380865467
NCBI Gene ID:	2994
NCBI Accession:	P06028.3
UniProt Secondary Accession:	P06028,Q0VAF4, Q58HE9, Q58HF0, Q58HF1, Q9UCH7, B8Q174 E2QBW7,
UniProt Related Accession:	P06028
Molecular Weight:	6,352 Da
NCBI Full Name:	Glycophorin-B
NCBI Synonym Full Names:	glycophorin B (MNS blood group)
NCBI Official Symbol:	GYPB
NCBI Official Synonym Symbols:	SS; GPB; GYP; MNS; PAS-3; CD235b
NCBI Protein Information:	glycophorin-B
UniProt Protein Name:	Glycophorin-B
UniProt Synonym Protein Names:	PAS-3; SS-active sialoglycoprotein; Sialoglycoprotein delta; CD_antigen: CD235b
Protein Family:	Glycophorin
UniProt Gene Name:	GYPB
UniProt Entry Name:	GLPB_HUMAN

Western blot analysis of extracts of Human serum, using GYPB Rabbit pAb (CAB7682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

Anti-GYPB Antibody (CAB15063)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)