Antibody Name:	Anti-GTF2B Antibody
Antibody SKU:	CAB1708
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GTF2B (NP_001505.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse thymus, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GTF2B (NP_001505.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SEWR TFSN DKAT KDPS RVGD SQNP LLSD GDLS TMIG KGTG AASF DEFG NSKY QNRR TMSS SDRA MMNA FKEI TTMA DRIN LPRN IVDR TNNL FKQV YEQK S
Gene ID:	2959
Uniprot:	Q00403
Cellular Location:	Nucleus
Calculated MW:	34kDa
Observed MW:	32KDa

Synonyms:

GTF2B, TF2B, TFIIB

Background:

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

UniProt Protein Function:	GTF2B: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. Associates with TFIID-IIA (DA complex) to form TFIID-IIA- IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1. Interacts with Epstein-Barr virus EBNA2. Belongs to the TFIIB family.
UniProt Protein Details:	Protein type:Acetyltransferase; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: 1p22-p21 Cellular Component: nucleolus; nucleoplasm; nucleus Molecular Function:protein binding; thyroid hormone receptor binding; transcription factor binding Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
NCBI Summary:	This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
UniProt Code:	Q00403
NCBI GenInfo Identifier:	135629
NCBI Gene ID:	2959
NCBI Accession:	Q00403.1
UniProt Secondary Accession:	Q00403,Q5JS30, A8K1A7,
UniProt Related Accession:	Q00403
Molecular Weight:	34,833 Da
NCBI Full Name:	Transcription initiation factor IIB
NCBI Synonym Full Names:	general transcription factor IIB
NCBI Official Symbol:	GTF2B
NCBI Official Synonym Symbols:	TF2B; TFIIB
NCBI Protein Information:	transcription initiation factor IIB
UniProt Protein Name:	Transcription initiation factor IIB
UniProt Synonym Protein Names:	General transcription factor TFIIB; S300-II
Protein Family:	Transcription initiation factor
UniProt Gene Name:	GTF2B
UniProt Entry Name:	TF2B_HUMAN

	Western blot analysis of extracts of various cell lines, using GTF2B antibody (CAB1708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human lung cancer using GTF2B Rabbit pAb (CAB1708) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using GTF2B Rabbit pAb (CAB1708) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using GTF2B Rabbit pAb (CAB1708) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using GTF2B Rabbit pAb (CAB1708) at dilution of 1:100 (40x lens).