Antibody Name:	Anti-GSTM3 Antibody
Antibody SKU:	CAB7679
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 101-225 of human GSTM3 (NP_000840.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, Mouse liver, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 101-225 of human GSTM3 (NP_000840.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VDII ENQV MDFR TQLI RLCY SSDH EKLK PQYL EELP GQLK QFSM FLGK FSWF AGEK LTFV DFLT YDIL DQNR IFDP KCLD EFPN LKAF MCRF EALE KIAA YLQS DQFC KMPI NNKM AQWG NKPV C
Gene ID:	2947
Uniprot:	P21266
Cellular Location:	Cytoplasm
Calculated MW:	26kDa
Observed MW:	25kDa

Synonyms:

GSTM3, GST5, GSTB, GSTM3-3, GTM3

Background:

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	GSTM3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers. Belongs to the GST superfamily. Mu family.
UniProt Protein Details:	Protein type:Transferase; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cell development/differentiation; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18 Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:identical protein binding; protein homodimerization activity; enzyme binding; glutathione binding; glutathione transferase activity Biological Process: glutathione metabolic process; establishment of blood-nerve barrier; nitrobenzene metabolic process; response to estrogen stimulus; xenobiotic metabolic process; xenobiotic catabolic process
NCBI Summary:	Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]
UniProt Code:	P21266
NCBI GenInfo Identifier:	21264423
NCBI Gene ID:	2947
NCBI Accession:	P21266.3
UniProt Related Accession:	P21266
Molecular Weight:	27kDa
NCBI Full Name:	Glutathione S-transferase Mu 3
NCBI Synonym Full Names:	glutathione S-transferase mu 3
NCBI Official Symbol:	GSTM3
NCBI Official Synonym Symbols:	GST5; GSTB; GTM3; GSTM3-3
NCBI Protein Information:	glutathione S-transferase Mu 3
UniProt Protein Name:	Glutathione S-transferase Mu 3
UniProt Synonym Protein Names:	GST class-mu 3; GSTM3-3; hGSTM3-3
Protein Family:	Glutathione S-transferase
UniProt Gene Name:	GSTM3
UniProt Entry Name:	GSTM3_HUMAN

	Western blot analysis of extracts of various cell lines, using GSTM3 antibody (CAB7679) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using GSTM3 antibody (CAB7679) at dilution of 1:100. Blue: DAPI for nuclear staining.