Antibody Name:	Anti-GSS Antibody [KO Validated]
Antibody SKU:	CAB14535
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, K-562, 293T, Mouse kidney, Mouse spleen, Mouse brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NLYG EEMV QALK QLKD SEER ASYI LMEK IEPE PFEN CLLR PGSP ARVV QCIS ELGI FGVY VRQE KTLV MNKH VGHL LRTK AIEH ADGG VAAG VAVL DNPY PV
Gene ID:	2937
Uniprot:	P48637
Cellular Location:
Calculated MW:	40kDa/52kDa
Observed MW:	54kDa

Synonyms:

GSS, GSHS, HEL-S-64p, HEL-S-88n

Background:

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

UniProt Protein Function:	GSS: Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5- oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. Belongs to the eukaryotic GSH synthase family.
UniProt Protein Details:	Protein type:EC 6.3.2.3; Other Amino Acids Metabolism - glutathione; Ligase Chromosomal Location of Human Ortholog: 20q11.2 Cellular Component: cytosol Molecular Function:protein homodimerization activity; glutathione binding; glycine binding; magnesium ion binding; glutathione synthase activity; ATP binding Biological Process: amino acid metabolic process; response to nutrient levels; nervous system development; response to cadmium ion; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; response to amino acid stimulus; aging Disease: Glutathione Synthetase Deficiency; Glutathione Synthetase Deficiency Of Erythrocytes, Hemolytic Anemia Due To
NCBI Summary:	Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:	P48637
NCBI GenInfo Identifier:	1346191
NCBI Gene ID:	2937
NCBI Accession:	P48637.1
UniProt Secondary Accession:	P48637,Q4TTD9, B2R697, B6F210, E1P5P9,
UniProt Related Accession:	P48637
Molecular Weight:	474
NCBI Full Name:	Glutathione synthetase
NCBI Synonym Full Names:	glutathione synthetase
NCBI Official Symbol:	GSS
NCBI Official Synonym Symbols:	GSHS; HEL-S-64p; HEL-S-88n
NCBI Protein Information:	glutathione synthetase; GSH-S; GSH synthetase; glutathione synthase; epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n
UniProt Protein Name:	Glutathione synthetase
UniProt Synonym Protein Names:	Glutathione synthase
Protein Family:	Glutathione synthetase
UniProt Gene Name:	GSS
UniProt Entry Name:	GSHB_HUMAN

	Western blot analysis of extracts of various cell lines, using GSS antibody (CAB14535) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Western blot analysis of extracts from normal (control) and GSS knockout (KO) 293T cells, using GSS antibody (CAB14535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies	ELISA Kits
Anti-GSS Antibody (CAB1069)	Human GSS (Glutathione synthetase) ELISA Kit (HUFI03449)
Anti-GSS Antibody (CAB13495)
Anti-Glutathione Synthetase Antibody (CAB11557)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)