Anti-GRSF1 Antibody (CAB20879)
- SKU:
- CAB20879
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-GRSF1 Antibody (CAB20879) |
| Antibody SKU: | CAB20879 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence, Immunoprecipitation |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human GRSF1. |
| Application: | Western blotting, Immunofluorescence, Immunoprecipitation |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HeLa, 293T, Human plasma |
| Immunogen: | Recombinant protein of Human GRSF1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | |
| Calculated MW: | 53kDa |
| Observed MW: | 53kDa |
| Synonyms: | GRSF1 |
| Background: | The protein encoded by this gene is a cellular protein that binds RNAs containing the G-rich element. The protein is localized in the cytoplasm, and has been shown to stimulate translation of viral mRNAs in vitro. Multiple transcript variants encoding different isoforms have been found for this gene. |
 | Immunofluorescence analysis of HeLa cells using GRSF1 Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug GRSF1 antibody . Western blot was performed from the immunoprecipitate using GRSF1 antibody at a dilition of 1:1000. |
 | Western blot analysis of extracts of various cell lines, using GRSF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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