KO Validated Antibodies 2

Anti-GRK2 [KO Validated] Antibody (CAB4443)

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SKU:
CAB4443
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
ADRBK1
Synonyms:
BARK1
Synonyms:
BETA-ARK1
Research Area:
Cell Biology

Description

Product Name:[KO Validated] GRK2 Rabbit mAb
Product Code:CAB4443
Size:20uL, 50uL, 100uL
Synonyms:ADRBK1, BARK1, BETA-ARK1
Applications:WB, IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human GRK2
Applications:WB, IF
Recommended Dilutions:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Mouse brain, Mouse spleen, Rat brain
Immunogen:A synthesized peptide derived from human GRK2
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:156
Uniprot:P25098
Cellular Location:Cell membrane, Cytoplasm
Calculated MW:80kDa
Observed MW:80KDa
UniProt Protein Function:GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function:G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; fibroblast growth factor receptor signaling pathway; acetylcholine receptor signaling, muscarinic pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; positive regulation of catecholamine secretion; innate immune response; cardiac muscle contraction

NCBI Summary:The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
UniProt Code:P25098
NCBI GenInfo Identifier:126302521
NCBI Gene ID:156
NCBI Accession:P25098.2
UniProt Secondary Accession:P25098,Q13837, Q6GTT3, B0ZBE1,
UniProt Related Accession:P25098
Molecular Weight:
NCBI Full Name:Beta-adrenergic receptor kinase 1
NCBI Synonym Full Names:adrenergic, beta, receptor kinase 1
NCBI Official Symbol:ADRBK1  
NCBI Official Synonym Symbols:GRK2; BARK1; BETA-ARK1  
NCBI Protein Information:beta-adrenergic receptor kinase 1; beta-ARK-1; G-protein coupled receptor kinase 2
UniProt Protein Name:Beta-adrenergic receptor kinase 1
UniProt Synonym Protein Names:G-protein coupled receptor kinase 2
UniProt Gene Name:ADRBK1  
UniProt Entry Name:ARBK1_HUMAN
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