Cell Biology Antibodies 6

Anti-GRAP Antibody (CAB15391)

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SKU:
CAB15391
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GRAP Antibody
Antibody SKU:CAB15391
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 128-217 of human GRAP (NP_006604.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:U-251MG, A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 128-217 of human GRAP (NP_006604.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LNEL VDFY RTTT IAKK RQIF LRDE EPLL KSPG ACFA QAQF DFSA QDPS QLSF RRGD IIEV LERP DPHW WRGR SCGR VGFF PRSY VQPV HL
Gene ID:10750
Uniprot:Q13588
Cellular Location:
Calculated MW:25kDa
Observed MW:36kDa
Synonyms:GRAP
Background:This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
NCBI Summary:This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway. [provided by RefSeq, Jul 2012]
UniProt Code:Q13588
NCBI GenInfo Identifier:5729856
NCBI Gene ID:10750
NCBI Accession:NP_006604.1
UniProt Related Accession:Q13588
Molecular Weight:36kDa
NCBI Full Name:GRB2-related adapter protein isoform 1
NCBI Synonym Full Names:GRB2 related adaptor protein
NCBI Official Symbol:GRAP  
NCBI Official Synonym Symbols:DFNB114  
NCBI Protein Information:GRB2-related adapter protein
UniProt Protein Name:GRB2-related adapter protein
Protein Family:GRB2-related adapter protein
UniProt Gene Name:GRAP  
UniProt Entry Name:GRAP_HUMAN
Anti-GRAP Antibody (CAB15391)Western blot analysis of extracts of various cell lines, using GRAP antibody (CAB15391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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