Cell Biology Antibodies 16

Anti-GPX4 Antibody (CAB11243)

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SKU:
CAB11243
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GPX4 Antibody
Antibody SKU:CAB11243
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human GPX4
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:PC-3, Jurkat, Mouse kidney, Mouse heart, Rat kidney
Immunogen:A synthesized peptide derived from human GPX4
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2879
Uniprot:P36969
Cellular Location:Cytoplasm, Mitochondrion
Calculated MW:17kDa
Observed MW:17KDa
Anti-GPX4 AntibodyWestern blot - GPX4 Rabbit mAb (CAB11243)
Synonyms:GPx-4, GSHPx-4, MCSP, PHGPx, SMDS, snGPx, snPHGPx
Background:The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016]
UniProt Protein Function:GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - glutathione; Nuclear envelope; Lipid Metabolism - arachidonic acid; EC 1.11.1.12; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; nucleus; cytosol

Molecular Function:selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity

Biological Process: glutathione metabolic process; lipoxygenase pathway; hydrogen peroxide catabolic process; multicellular organismal development; phospholipid metabolic process; regulation of inflammatory response; arachidonic acid metabolic process; response to oxidative stress; response to estradiol stimulus; aging

NCBI Summary:This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:P36969
NCBI GenInfo Identifier:172045844
NCBI Gene ID:2879
NCBI Accession:P36969.3
UniProt Secondary Accession:P36969,O43381, Q6PJ59, Q9UPK2,
UniProt Related Accession:P36969
Molecular Weight:Observed MW: 22kDaCalculated MW: 19kDa/22kDa
NCBI Full Name:Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
NCBI Synonym Full Names:glutathione peroxidase 4
NCBI Official Symbol:GPX4  
NCBI Official Synonym Symbols:MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx  
NCBI Protein Information:phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
UniProt Protein Name:Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
UniProt Synonym Protein Names:Glutathione peroxidase 4; GPx-4; GSHPx-4
Protein Family:Probable glutathione peroxidase
UniProt Gene Name:GPX4  
UniProt Entry Name:GPX4_HUMAN
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