Name: Anti-GPX3 Antibody (CAB12956)
Brand: Cell Biology Antibodies 3
SKU: CAB12956
Availability: OutOfStock

Antibody Name:	Anti-GPX3 Antibody
Antibody SKU:	CAB12956
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human GPX3.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse kidney, Mouse liver

Immunogen:	Recombinant protein of human GPX3.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	2878
Uniprot:	P22352
Cellular Location:	Secreted
Calculated MW:	25kDa
Observed MW:	25kDa

Synonyms:

GPX3, GPx-P, GSHPx-3, GSHPx-P

Background:

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

UniProt Protein Function:	GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.
UniProt Protein Details:	Protein type:Secreted; Other Amino Acids Metabolism - glutathione; Oxidoreductase; Secreted, signal peptide; Lipid Metabolism - arachidonic acid; EC 1.11.1.9 Chromosomal Location of Human Ortholog: 5q33.1 Cellular Component: extracellular region; extracellular space Molecular Function:glutathione peroxidase activity; protein binding; selenium binding; transcription factor binding Biological Process: hydrogen peroxide catabolic process; protein homotetramerization; response to lipid hydroperoxide; response to reactive oxygen species
NCBI Summary:	This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
UniProt Code:	P22352
NCBI GenInfo Identifier:	172046796
NCBI Gene ID:	2878
NCBI Accession:	P22352.2
UniProt Secondary Accession:	P22352,O43787, Q86W78, Q9NZ74, Q9UEL1,
UniProt Related Accession:	P22352
Molecular Weight:	25,552 Da
NCBI Full Name:	Glutathione peroxidase 3
NCBI Synonym Full Names:	glutathione peroxidase 3
NCBI Official Symbol:	GPX3
NCBI Official Synonym Symbols:	GPx-P; GSHPx-3; GSHPx-P
NCBI Protein Information:	glutathione peroxidase 3
UniProt Protein Name:	Glutathione peroxidase 3
UniProt Synonym Protein Names:	Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P
Protein Family:	Probable glutathione peroxidase
UniProt Gene Name:	GPX3
UniProt Entry Name:	GPX3_HUMAN

Western blot analysis of extracts of various cell lines, using GPX3 antibody (CAB12956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human Glutathione Peroxidase 3 / GPX3 ELISA Kit

Human Glutathione peroxidase 3 (GPX3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)