Name: Anti-GPSM1 Antibody (CAB8713)
Brand: Developmental Biology
SKU: CAB8713
Availability: OutOfStock

Antibody Name:	Anti-GPSM1 Antibody
Antibody SKU:	CAB8713
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 158-457 of human GPSM1 (NP_056412.5).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, HT-29, MCF7, Jurkat, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 158-457 of human GPSM1 (NP_056412.5).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AKGK QLSW NAAN ATQD PGHL PPDV RETL CKAS EFYE RNLS LVKE LGDR AAQG RAYG NLGN THYL LGNF TEAT TFHK ERLA IAKE FGDK AAER RAYS NLGN AHVF LGRF DVAA EYYK KTLQ LSRQ LRDQ AVEA QACY SLGN TYTL LQDY ERAA EYHL RHLL IAQE LADR VGEG RACW SLGN AYVS MGRP AQAL TFAK KHLQ ISQE IGDR HGEL TARM NVAQ LQLV LGRL TSPA ASEK PDLA GYEA QGEF QGCG GVLL PTGT DRIR SCGG VGSR PGQH GGGG SRQK MAPT SQFF LASG TAQA
Gene ID:	26086
Uniprot:	Q86YR5
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein, cytosol
Calculated MW:	18kDa/49kDa/72kDa/74kDa
Observed MW:	72kDa

Synonyms:

GPSM1, AGS3

Background:

G-protein signaling modulators (GPSMs) play diverse functional roles through their interaction with G-protein subunits. This gene encodes a receptor-independent activator of G protein signaling, which is one of several factors that influence the basal activity of G-protein signaling systems. The protein contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GPSM1: Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G- protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction. Belongs to the GPSM family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GEFs; GEFs, misc. Chromosomal Location of Human Ortholog: 9q34.3 Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; plasma membrane; cytosol Molecular Function:GDP-dissociation inhibitor activity Biological Process: nervous system development; regulation of catalytic activity; cell differentiation; regulation of G-protein coupled receptor protein signaling pathway
NCBI Summary:	G-protein signaling modulators (GPSMs) play diverse functional roles through their interaction with G-protein subunits. This gene encodes a receptor-independent activator of G protein signaling, which is one of several factors that influence the basal activity of G-protein signaling systems. The protein contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q86YR5
NCBI GenInfo Identifier:	294862435
NCBI Gene ID:	26086
NCBI Accession:	Q86YR5.2
UniProt Secondary Accession:	Q86YR5,Q86SR5, Q969T1, Q9UFS8, A9Z1X4, B1B0W3,
UniProt Related Accession:	Q86YR5
Molecular Weight:	72,136 Da
NCBI Full Name:	G-protein-signaling modulator 1
NCBI Synonym Full Names:	G-protein signaling modulator 1
NCBI Official Symbol:	GPSM1
NCBI Official Synonym Symbols:	AGS3
NCBI Protein Information:	G-protein-signaling modulator 1; activator of G-protein signaling 3; G-protein signalling modulator 1 (AGS3-like, C. elegans)
UniProt Protein Name:	G-protein-signaling modulator 1
UniProt Synonym Protein Names:	Activator of G-protein signaling 3
Protein Family:	G-protein-signaling modulator
UniProt Gene Name:	GPSM1
UniProt Entry Name:	GPSM1_HUMAN

Anti-GPSM1 Polyclonal Antibody (CAB8713)

Western blot analysis of extracts of various cell lines, using GPSM1 antibody (CAB8713) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

GPSM1 Antibody (PACO03814)

GPSM1 Antibody (PACO09573)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)