Name: Anti-GPR55 Antibody (CAB12890)
Brand: Cell Biology Antibodies 3
SKU: CAB12890
Availability: OutOfStock

Antibody Name:	Anti-GPR55 Antibody
Antibody SKU:	CAB12890
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-319 of human GPR55 (NP_005674.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-319 of human GPR55 (NP_005674.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GRRD HTQD WVQQ KACI YSIA ASLA VFVV SFLP VHLG FFLQ FLVR NSFI VECR AKQS ISFF LQLS MCFS NVNC CLDV FCYY FVIK EFRM NIRA HRPS RVQL VLQD TTIS RG
Gene ID:	9290
Uniprot:	Q9Y2T6
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	36kDa
Observed MW:	37kDa

Synonyms:	GPR55, LPIR1
Background:	This gene belongs to the G-protein-coupled receptor superfamily. The encoded integral membrane protein is a likely cannabinoid receptor. It may be involved in several physiological and pathological processes by activating a variety of signal transduction pathways.

UniProt Protein Function:	GPR55: May be involved in hyperalgesia associated with inflammatory and neuropathic pain. Receptor for L- alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:	Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:cannabinoid receptor activity; G-protein coupled receptor activity Biological Process: bone resorption; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein coupled receptor protein signaling pathway; negative regulation of osteoclast differentiation; phospholipase C activation; positive regulation of Rho protein signal transduction
NCBI Summary:	This gene belongs to the G-protein-coupled receptor superfamily. The encoded integral membrane protein is a likely cannabinoid receptor. It may be involved in several physiological and pathological processes by activating a variety of signal transduction pathways. [provided by RefSeq, Aug 2013]
UniProt Code:	Q9Y2T6
NCBI GenInfo Identifier:	71159390
NCBI Gene ID:	9290
NCBI Accession:	Q9Y2T6.2
UniProt Secondary Accession:	Q9Y2T6,Q8N580,
UniProt Related Accession:	Q9Y2T6
Molecular Weight:	Calculated MW: 36kDaObserved MW: 37kDa
NCBI Full Name:	G-protein coupled receptor 55
NCBI Synonym Full Names:	G protein-coupled receptor 55
NCBI Official Symbol:	GPR55
NCBI Official Synonym Symbols:	LPIR1
NCBI Protein Information:	G-protein coupled receptor 55
UniProt Protein Name:	G-protein coupled receptor 55
Protein Family:	G-protein coupled receptor
UniProt Gene Name:	GPR55

	Western blot analysis of extracts of various cell lines, using GPR55 antibody (CAB12890) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of rat bone marrow cells using GPR55 antibody (CAB12890) at dilution of 1:100. Blue: DAPI for nuclear staining.