Cell Biology Antibodies 2
Anti-GPN1 Antibody (CAB12167)
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- SKU:
- CAB12167
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GPN1 Antibody |
| Antibody SKU: | CAB12167 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 269-388 of human GPN1 (NP_009197.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse lung, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 269-388 of human GPN1 (NP_009197.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FVQV TSAA EEYE REYR PEYE RLKK SLAN AESQ QQRE QLER LRKD MGSV ALDA GTAK DSLS PVLH PSDL ILTR GTLD EEDE EADS DTDD IDHR VTEE SHEE PAFQ NFMQ ESMA QYWK RNNK |
| Gene ID: | 11321 |
| Uniprot: | Q9HCN4 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 31kDa/33kDa/40kDa/41kDa/43kDa |
| Observed MW: | 42kDa |
| Synonyms: | GPN1, ATPBD1A, MBDIN, NTPBP, RPAP4, XAB1 |
| Background: | This gene encodes a guanosine triphosphatase enzyme. The encoded protein may play a role in DNA repair and may function in activation of transcription. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | GPN1: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. May be involved in nuclear localization of XPA. Belongs to the GPN-loop GTPase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Hydrolase Chromosomal Location of Human Ortholog: 2p23.3 Cellular Component: cytoplasm; nucleus Molecular Function:GTPase activity; protein binding; GTP binding Biological Process: metabolic process |
| UniProt Code: | Q9HCN4 |
| NCBI GenInfo Identifier: | 34925430 |
| NCBI Gene ID: | |
| NCBI Accession: | Q9HCN4.1 |
| Molecular Weight: | |
| NCBI Full Name: | GPN-loop GTPase 1 |
| UniProt Protein Name: | GPN-loop GTPase 1 |
| UniProt Synonym Protein Names: | MBD2-interacting protein; XPA-binding protein 1 |
| UniProt Gene Name: | GPN1 |
| UniProt Entry Name: | GPN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPN1 antibody (CAB12167) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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