Anti-GPBAR1 Antibody (CAB20778)
- SKU:
- CAB20778
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Rabbit
- Isotype:
- Horseradish peroxidase conjugated IgG
Description
| Antibody Name: | Anti-GPBAR1 Antibody (CAB20778) |
| Antibody SKU: | CAB20778 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 231-330 of human GPBAR1 (NP_733800.1). |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | Rat liver |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 231-330 of human GPBAR1 (NP_733800.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLFG LCWG PYVA TLLL SVLA YEQR PPLG PGTL LSLL SLGS ASAA AVPV AMGL GDQR YTAP WRAA AQRC LQGL WGRA SRDS PGPS IAYH PSSQ SSVD LDLN |
| Cellular Location: | |
| Calculated MW: | 35kDa |
| Observed MW: | 35KDa |
| Synonyms: | BG37, TGR5, M-BAR, GPCR19, GPR131 |
| Background: | This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. |
 | Immunofluorescence analysis of HCT116 cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HepG2 cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of 293T cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of Rat liver, using GPBAR1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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