Name: Anti-GNAQ Antibody (CAB14736)
Brand: Cell Biology Antibodies 5
SKU: CAB14736
Availability: OutOfStock

Antibody Name:	Anti-GNAQ Antibody
Antibody SKU:	CAB14736
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-359 of human GNAQ (NP_002063.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, OVCAR3, HT-29, U-251MG, LO2, Mouse brain, Mouse heart, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-359 of human GNAQ (NP_002063.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTLE SIMA CCLS EEAK EARR INDE IERQ LRRD KRDA RREL KLLL LGTG ESGK STFI KQMR IIHG SGYS DEDK RGFT KLVY QNIF TAMQ AMIR AMDT LKIP YKYE HNKA HAQL VREV DVEK VSAF ENPY VDAI KSLW NDPG IQEC YDRR REYQ LSDS TKYY LNDL DRVA DPAY LPTQ QDVL RVRV PTTG IIEY PFDL QSVI FRMV DVGG QRSE RRKW IHCF ENVT SIMF LVAL SEYD QVLV ESDN ENRM EESK ALFR TIIT YPWF QNSS VILF LNKK DLLE EKIM YSHL VDYF PEYD GPQR DAQA AREF ILKM FVDL NPDS DKII YSHF TCAT DTEN IRFV FAAV KDTI LQLN LKEY NLV
Gene ID:	2776
Uniprot:	P50148
Cellular Location:	Membrane, Nucleus, Nucleus membrane
Calculated MW:	42kDa
Observed MW:	42kDa

Synonyms:	GNAQ, CMC1, G-ALPHA-q, GAQ, SWS
Background:	This locus encodes a guanine nucleotide-binding protein. The encoded protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospolipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation. A related pseudogene exists on chromosome 2.

UniProt Protein Function:	G-alpha(q): a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
UniProt Protein Details:	Protein type:G protein, heterotrimeric; G protein; G protein, heterotrimeric alpha G(q) Chromosomal Location of Human Ortholog: 9q21 Cellular Component: cytoplasm; lysosomal membrane; photoreceptor outer segment; plasma membrane Molecular Function:GTPase activator activity; GTPase activity; protein binding; type 2A serotonin receptor binding Biological Process: acetylcholine receptor signaling, muscarinic pathway; blood coagulation; dopamine receptor, phospholipase C activating pathway; entrainment of circadian clock; G-protein signaling, adenylate cyclase activating pathway; glutamate signaling pathway; negative regulation of protein kinase activity; phospholipase C activation; phototransduction, visible light; platelet activation; protein stabilization; regulation of action potential Disease: Capillary Malformations, Congenital; Sturge-weber Syndrome
NCBI Summary:	This locus encodes a guanine nucleotide-binding protein. The encoded protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospolipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation. A related pseudogene exists on chromosome 2.[provided by RefSeq, Nov 2010]
UniProt Code:	P50148
NCBI GenInfo Identifier:	251757492
NCBI Gene ID:	2776
NCBI Accession:	P50148.4
UniProt Secondary Accession:	P50148,O15108, Q13462, Q6NT27, Q92471, Q9BZB9,
UniProt Related Accession:	P50148
Molecular Weight:	42,142 Da
NCBI Full Name:	Guanine nucleotide-binding protein G(q) subunit alpha
NCBI Synonym Full Names:	G protein subunit alpha q
NCBI Official Symbol:	GNAQ
NCBI Official Synonym Symbols:	GAQ; SWS; CMC1; G-ALPHA-q
NCBI Protein Information:	guanine nucleotide-binding protein G(q) subunit alpha
UniProt Protein Name:	Guanine nucleotide-binding protein G(q) subunit alpha
UniProt Synonym Protein Names:	Guanine nucleotide-binding protein alpha-q
UniProt Gene Name:	GNAQ
UniProt Entry Name:	GNAQ_HUMAN

	Western blot analysis of extracts of various cell lines, using GNAQ antibody (CAB14736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of L929 cells using GNAQ Rabbit pAb (CAB14736) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.