Antibody Name:	Anti-GNAI3 Antibody
Antibody SKU:	CAB15674
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, HepG2, Mouse kidney, Mouse brain, Rat kidney

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGCT LSAE DKAA VERS KMID RNLR EDGE KAAK EVKL LLLG AGES GKST IVKQ MKII HEDG YSED ECKQ YKVV VYSN TIQS IIAI IRAM GRLK IDFG EAAR
Gene ID:	2773
Uniprot:	P08754
Cellular Location:	Cell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	40kDa
Observed MW:	40kDa

Synonyms:

GNAI3, 87U6, ARCND1

Background:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.

UniProt Protein Function:	G-alpha i3: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor- regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Defects in GNAI3 are the cause of auriculocondylar syndrome 1 (ARCND1). ARCND1 is an autosomal dominant craniofacial malformation syndrome characterized by variable mandibular anomalies, including mild to severe micrognathia, temporomandibular joint ankylosis, cleft palate, and a characteristic ear malformation that consists of separation of the lobule from the external ear, giving the appearance of a question mark (question-mark ear). Other frequently described features include prominent cheeks, cupped and posteriorly rotated ears, preauricular tags, and microstomia. Belongs to the G-alpha family. G(i/o/t/z) subfamily.
UniProt Protein Details:	Protein type:G protein; G protein, heterotrimeric alpha G((i/o/t/z)); G protein, heterotrimeric Chromosomal Location of Human Ortholog: 1p13 Cellular Component: centrosome; cytoplasm; heterotrimeric G-protein complex; lysosomal membrane; membrane; midbody; plasma membrane Molecular Function:G-protein beta/gamma-subunit binding; G-protein-coupled receptor binding; GDP binding; GTP binding; GTPase activity; protein binding; signal transducer activity Biological Process: cell division; dopamine receptor signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; GTP metabolic process; negative regulation of adenylate cyclase activity; protein folding Disease: Auriculocondylar Syndrome 1
NCBI Summary:	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]
UniProt Code:	P08754
NCBI GenInfo Identifier:	120996
NCBI Gene ID:	2773
NCBI Accession:	P08754.3
UniProt Secondary Accession:	P08754,P17539, Q5TZX1,
UniProt Related Accession:	P08754
Molecular Weight:	40,532 Da
NCBI Full Name:	Guanine nucleotide-binding protein G(k) subunit alpha
NCBI Synonym Full Names:	G protein subunit alpha i3
NCBI Official Symbol:	GNAI3
NCBI Official Synonym Symbols:	87U6; ARCND1
NCBI Protein Information:	guanine nucleotide-binding protein G(k) subunit alpha
UniProt Protein Name:	Guanine nucleotide-binding protein G(k) subunit alpha
UniProt Synonym Protein Names:	G(i) alpha-3
UniProt Gene Name:	GNAI3
UniProt Entry Name:	GNAI3_HUMAN

	Western blot analysis of extracts of various cell lines, using GNAI3 antibody (CAB15674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using GNAI3 antibody (CAB15674) at dilution of 1:100. Blue: DAPI for nuclear staining.