Antibody Name:	Anti-GNAI3 Antibody [KO Validated]
Antibody SKU:	CAB13307
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	LO2, MCF-7, HL-60, HT-1080

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGCT LSAE DKAA VERS KMID RNLR EDGE KAAK EVKL LLLG AGES GKST IVKQ MKII HEDG YSED ECKQ YKVV VYSN TIQS IIAI IRAM GRLK IDFG EAAR ADDA RQLF VLAG SAEE GVMT PELA GVIK RLWR DGGV QACF SRSR EYQL NDSA SYYL NDLD RISQ SNYI PTQQ DVLR TRVK TTGI VETH FTFK DLYF KMFD VGGQ RSER KKWI HCFE GVTA IIFC VALS DYDL VLAE DEEM NRMH ESMK LFDS ICNN KWFT ETSI ILFL NKKD LFEE KIKR SPLT ICYP EYTG SNTY EEAA AYIQ CQFE DLNR RKDT KEIY THFT CATD TKNV QFVF DAVT DVII KNNL KECG LY
Gene ID:	2773
Uniprot:	P08754
Cellular Location:	Cell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	40kDa
Observed MW:	45kDa

Synonyms:

GNAI3, 87U6, ARCND1

Background:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.

UniProt Protein Function:	G-alpha i3: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor- regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Defects in GNAI3 are the cause of auriculocondylar syndrome 1 (ARCND1). ARCND1 is an autosomal dominant craniofacial malformation syndrome characterized by variable mandibular anomalies, including mild to severe micrognathia, temporomandibular joint ankylosis, cleft palate, and a characteristic ear malformation that consists of separation of the lobule from the external ear, giving the appearance of a question mark (question-mark ear). Other frequently described features include prominent cheeks, cupped and posteriorly rotated ears, preauricular tags, and microstomia. Belongs to the G-alpha family. G(i/o/t/z) subfamily.
UniProt Protein Details:	Protein type:G protein; G protein, heterotrimeric alpha G((i/o/t/z)); G protein, heterotrimeric Chromosomal Location of Human Ortholog: 1p13 Cellular Component: centrosome; cytoplasm; heterotrimeric G-protein complex; lysosomal membrane; membrane; midbody; plasma membrane Molecular Function:G-protein beta/gamma-subunit binding; G-protein-coupled receptor binding; GDP binding; GTP binding; GTPase activity; protein binding; signal transducer activity Biological Process: cell division; dopamine receptor signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; GTP metabolic process; negative regulation of adenylate cyclase activity; protein folding Disease: Auriculocondylar Syndrome 1
NCBI Summary:	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]
UniProt Code:	P08754
NCBI GenInfo Identifier:	120996
NCBI Gene ID:	2773
NCBI Accession:	P08754.3
UniProt Secondary Accession:	P08754,P17539, Q5TZX1,
UniProt Related Accession:	P08754
Molecular Weight:	40,532 Da
NCBI Full Name:	Guanine nucleotide-binding protein G(k) subunit alpha
NCBI Synonym Full Names:	G protein subunit alpha i3
NCBI Official Symbol:	GNAI3
NCBI Official Synonym Symbols:	87U6; ARCND1
NCBI Protein Information:	guanine nucleotide-binding protein G(k) subunit alpha
UniProt Protein Name:	Guanine nucleotide-binding protein G(k) subunit alpha
UniProt Synonym Protein Names:	G(i) alpha-3
UniProt Gene Name:	GNAI3
UniProt Entry Name:	GNAI3_HUMAN

	Western blot analysis of extracts of various cell lines, using GNAI3 antibody (CAB13307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and GNAI3 knockout (KO) HeLa cells, using GNAI3 antibody (CAB13307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of L929 cells using [KO Validated] GNAI3 Rabbit pAb (CAB13307) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GNAI3 antibody (CAB13307). Western blot was performed from the immunoprecipitate using GNAI3 antibody (CAB13307) at a dilition of 1:1000.