Anti-Glyt2 Antibody (CAB20931)

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SKU:
CAB20931
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-Glyt2 Antibody (CAB20931)
Antibody SKU:CAB20931
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human Glyt2.
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-937, SH-SY5Y, Mouse spinal cord, Mouse brain, Mouse lung, Rat spinal cord, Rat brain
Immunogen:Recombinant protein of Human Glyt2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:87kDa
Observed MW:87KDa
Synonyms:SLC6A5, GLYT-2, GLYT2, HKPX3, NET1
Background:This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This integral membrane glycoprotein is responsible for the clearance of extracellular glycine during glycine-mediated neurotransmission. This protein is found in glycinergic axons and maintains a high presynaptic pool of neurotransmitter at glycinergic synapses. Mutations in this gene cause hyperekplexia; a heterogenous neurological disorder characterized by exaggerated startle responses and neonatal apnea. Two transcript variants encoding different isoforms have been found for this gene.
Western blot analysis of extracts of various cell lines, using Glyt2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using Glyt2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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