Signal Transduction Antibodies 3

Anti-GLUT4 Antibody (CAB7637)

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SKU:
CAB7637
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-GLUT4 Antibody
Antibody SKU:CAB7637
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human GLUT4 (NP_001033.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3, HepG2, Mouse kidney, Mouse heart, Rat heart, Rat kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human GLUT4 (NP_001033.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GPIP WFIV AELF SQGP RPAA MAVA GFSN WTSN FIIG MGFQ YVAE AMGP YVFL LFAV LLLG FFIF TFLR VPET RGRT FDQI SAAF HRTP SLLE QEVK PSTE LEYL GPDE ND
Gene ID:6517
Uniprot:P14672
Cellular Location:Cell membrane, Cytoplasm, Endomembrane system, Multi-pass membrane protein, perinuclear region
Calculated MW:43kDa/54kDa
Observed MW:54kDa
Synonyms:SLC2A4, GLUT4
Background:This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
UniProt Protein Function:GLUT4: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Its surface expression is regulated by insulin. Has two internalization sequences, a dileucine repeat present in the C-terminus and a FxxY motif in the amino-terminal end. Associates with an intracellular tubulo-vesicular compartment under low plasma conditions. The binding of insulin to the insulin receptor (a typosine kinase) leads to the rapid translocation of GLUT4 to the cell surface, increasing cellular glucose transport activity. Its translocation to the plasma membrane is also stimulated by exercise, but independent from insulin signaling. This insulin-independent pathway may be regulated by AMPK.
UniProt Protein Details:

Protein type:Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: multivesicular body; trans-Golgi network transport vesicle; membrane; clathrin-coated vesicle; integral to plasma membrane; perinuclear region of cytoplasm; T-tubule; plasma membrane; endomembrane system; coated pit; vesicle membrane; cytosol; lipid raft; external side of plasma membrane

Molecular Function:D-glucose transmembrane transporter activity; protein binding; glucose transmembrane transporter activity

Biological Process: amylopectin biosynthetic process; response to ethanol; cellular response to insulin stimulus; glucose import; hexose transport; carbohydrate metabolic process; brown fat cell differentiation; pathogenesis; glucose transport; glucose homeostasis; transmembrane transport

Disease: Diabetes Mellitus, Noninsulin-dependent

NCBI Summary:This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]
UniProt Code:P14672
NCBI GenInfo Identifier:121761
NCBI Gene ID:6517
NCBI Accession:P14672.1
UniProt Secondary Accession:P14672,Q05BQ3, Q14CX2,
UniProt Related Accession:P14672
Molecular Weight:509
NCBI Full Name:Solute carrier family 2, facilitated glucose transporter member 4
NCBI Synonym Full Names:solute carrier family 2 (facilitated glucose transporter), member 4
NCBI Official Symbol:SLC2A4  
NCBI Official Synonym Symbols:GLUT4  
NCBI Protein Information:solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; insulin-responsive glucose transporter type 4; glucose transporter type 4, insulin-responsive
UniProt Protein Name:Solute carrier family 2, facilitated glucose transporter member 4
UniProt Synonym Protein Names:Glucose transporter type 4, insulin-responsive; GLUT-4
UniProt Gene Name:SLC2A4  
UniProt Entry Name:GTR4_HUMAN
Anti-GLUT4 Antibody (CAB7637)Western blot analysis of extracts of various cell lines, using GLUT4 antibody (CAB7637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-GLUT4 Antibody (CAB7637)Immunohistochemistry of paraffin-embedded mouse skeletal muscle using GLUT4 Rabbit pAb (CAB7637) at dilution of 1:100 (40x lens).
Anti-GLUT4 Antibody (CAB7637)Immunofluorescence analysis of Mouse skeletal muscle using GLUT4 Rabbit pAb (CAB7637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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