Cell Biology Antibodies 6

Anti-GLS2 Antibody (CAB16029)

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SKU:
CAB16029
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-GLS2 Antibody
Antibody SKU:CAB16029
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human GLS2
Application:WB IF
Recommended Dilution:WB 1:500 - 1:1000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:
Immunogen:A synthetic peptide of human GLS2
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:27165
Uniprot:Q9UI32
Cellular Location:Mitochondrion
Calculated MW:23kDa/66kDa
Observed MW:66kDa
Synonyms:GLS2, GA, GLS, LGA, hLGA
Background:The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms.
UniProt Protein Function:GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; EC 3.5.1.2; Energy Metabolism - nitrogen; Hydrolase; Mitochondrial; Other Amino Acids Metabolism - D-Glutamine and D-glutamate

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function:glutaminase activity; protein binding

Biological Process: amino acid biosynthetic process; amino acid metabolic process; glutamate biosynthetic process; glutamate secretion; glutamine catabolic process; regulation of apoptosis

NCBI Summary:The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
UniProt Code:Q9UI32
NCBI GenInfo Identifier:145559477
NCBI Gene ID:27165
NCBI Accession:Q9UI32.2
UniProt Secondary Accession:Q9UI32,Q8IX91, Q9NYY2, Q9UI31, B7Z8Q9,
UniProt Related Accession:Q9UI32
Molecular Weight:66kDa
NCBI Full Name:Glutaminase liver isoform, mitochondrial
NCBI Synonym Full Names:glutaminase 2
NCBI Official Symbol:GLS2  
NCBI Official Synonym Symbols:GA; GLS; LGA; hLGA  
NCBI Protein Information:glutaminase liver isoform, mitochondrial
UniProt Protein Name:Glutaminase liver isoform, mitochondrial
UniProt Synonym Protein Names:L-glutaminase; L-glutamine amidohydrolase
Protein Family:Glucosidase
UniProt Gene Name:GLS2  
Anti-GLS2 Antibody (CAB16029)Western blot analysis of extracts of various cell lines, using GLS2 antibody (CAB16029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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