Name: Anti-GLRB Antibody (CAB10505)
Brand: Signal Transduction Antibodies 1
SKU: CAB10505
Availability: OutOfStock

Antibody Name:	Anti-GLRB Antibody
Antibody SKU:	CAB10505
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-265 of human GLRB (NP_000815.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, NCI-H460, HT-1080, THP-1, Mouse brain, Mouse liver, Mouse eye, Rat brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-265 of human GLRB (NP_000815.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KEKS SKKG KGKK KQYL CPSQ QSAE DLAR VPAN STSN ILNR LLVS YDPR IRPN FKGI PVDV VVNI FINS FGSI QETT MDYR VNIF LRQK WNDP RLKL PSDF RGSD ALTV DPTM YKCL WKPD LFFA NEKS ANFH DVTQ ENIL LFIF RDGD VLVS MRLS ITLS CPLD LTLF PMDT QRCK MQLE SFGY TTDD LRFI WQSG DPVQ LEKI ALPQ FDIK KEDI EYGN CTKY YKGT GYYT CVEV IFTL RRQ
Gene ID:	2743
Uniprot:	P48167
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	34kDa/56kDa
Observed MW:	58kDa

Synonyms:

GLRB, HKPX2

Background:

This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	GLRB: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). Defects in GLRB are the cause of hyperekplexia type 2 (HKPX2). HKPX2 is a neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile2 stimuli. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRB sub- subfamily.
UniProt Protein Details:	Protein type:Transporter, ion channel; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral; Transporter Chromosomal Location of Human Ortholog: 4q31.3 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:extracellular-glycine-gated chloride channel activity; extracellular-glycine-gated ion channel activity; glycine binding; protein binding Biological Process: ion transport; nervous system development; neuropeptide signaling pathway; response to amino acid stimulus; startle response; synaptic transmission; synaptic transmission, glycinergic Disease: Hyperekplexia 2
NCBI Summary:	This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Code:	P48167
NCBI GenInfo Identifier:	1346173
NCBI Gene ID:	2743
NCBI Accession:	P48167.1
UniProt Secondary Accession:	P48167,A8K3K2, D3DP23, F5GWE1,
UniProt Related Accession:	P48167
Molecular Weight:	34,944 Da
NCBI Full Name:	Glycine receptor subunit beta
NCBI Synonym Full Names:	glycine receptor beta
NCBI Official Symbol:	GLRB
NCBI Official Synonym Symbols:	HKPX2
NCBI Protein Information:	glycine receptor subunit beta
UniProt Protein Name:	Glycine receptor subunit beta
UniProt Synonym Protein Names:	Glycine receptor 58 kDa subunit
Protein Family:	Glycine receptor
UniProt Gene Name:	GLRB
UniProt Entry Name:	GLRB_HUMAN

Western blot analysis of extracts of various cell lines, using GLRB antibody (CAB10505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

GLRB Antibody (PACO03905)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)