Signal Transduction Antibodies 2

Anti-GLRA2 Antibody (CAB15673)

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SKU:
CAB15673
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-GLRA2 Antibody
Antibody SKU:CAB15673
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 350-420 of human GLRA2 (NP_002054.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse spinal cord, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 350-420 of human GLRA2 (NP_002054.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RLRR RQKR QNKE EDVT RESR FNFS GYGM GHCL QVKD GTAV KATP ANPL PQPP KDGD AIKK KFVD RAKR IDT
Gene ID:2742
Uniprot:P23416
Cellular Location:Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:42kDa/51kDa/52kDa
Observed MW:52kDa
Synonyms:GLRA2, GLR
Background:The glycine receptor consists of two subunits, alpha and beta, and acts as a pentamer. The protein encoded by this gene is an alpha subunit and can bind strychnine. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:GLRA2: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA2 sub- subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, ion channel

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function:transmitter-gated ion channel activity; extracellular-glycine-gated chloride channel activity; glycine binding

Biological Process: neuropeptide signaling pathway; ion transport; transmembrane transport

NCBI Summary:The glycine receptor consists of two subunits, alpha and beta, and acts as a pentamer. The protein encoded by this gene is an alpha subunit and can bind strychnine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]
UniProt Code:P23416
NCBI GenInfo Identifier:121578
NCBI Gene ID:2742
NCBI Accession:P23416.1
UniProt Secondary Accession:P23416,Q7TNC8, P22771,
UniProt Related Accession:P23416
Molecular Weight:452
NCBI Full Name:Glycine receptor subunit alpha-2
NCBI Synonym Full Names:glycine receptor, alpha 2
NCBI Official Symbol:GLRA2  
NCBI Official Synonym Symbols:GLR  
NCBI Protein Information:glycine receptor subunit alpha-2; glycine receptor alpha 2 subunit; glycine receptor, alpha-2 polypeptide
UniProt Protein Name:Glycine receptor subunit alpha-2
Protein Family:Glycine receptor
UniProt Gene Name:GLRA2  
UniProt Entry Name:GLRA2_HUMAN
Anti-GLRA2 Antibody (CAB15673)Western blot analysis of extracts of various cell lines, using GLRA2 antibody (CAB15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.
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