KO Validated Antibodies 1

Anti-GIT1 Antibody (CAB15437)[KO Validated]

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SKU:
CAB15437
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-GIT1 Antibody [KO Validated]
Antibody SKU:CAB15437
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2).
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, A-549, A375, Mouse heart, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EIHK LQAE NLQL RQPP GPVP TPPL PSER AEHT PMAP GGST HRRD RQAF SMYE PGSA LKPF GGPP GDEL TTRL QPFH STEL EDDA IYSV HVPA GLYR IRKG VSAS AVPF TPSS PLLS CSQE GSRH TSKL SRHG SGAD SDYE NTQS GDPL LGLE GKRF LELG KEED FHPE LESL DGDL DPGL P
Gene ID:28964
Uniprot:Q9Y2X7
Cellular Location:Cytoplasm
Calculated MW:19kDa/84kDa/85kDa
Observed MW:100kDa
Synonyms:GIT1
Background:
UniProt Protein Function:GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).
UniProt Protein Details:

Protein type:GAPs, ARF; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane

Molecular Function:protein binding

Biological Process: ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway

UniProt Code:Q9Y2X7
NCBI GenInfo Identifier:45645212
NCBI Gene ID:28964
NCBI Accession:Q9Y2X7.2
UniProt Secondary Accession:Q9Y2X7,Q86SS0, Q9BRJ4, B4DGU9, B4DSV3,
UniProt Related Accession:Q9Y2X7
Molecular Weight:85,446 Da
NCBI Full Name:ARF GTPase-activating protein GIT1
NCBI Synonym Full Names:GIT ArfGAP 1
NCBI Official Symbol:GIT1  
NCBI Protein Information:ARF GTPase-activating protein GIT1
UniProt Protein Name:ARF GTPase-activating protein GIT1
UniProt Synonym Protein Names:Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1
Protein Family:ARF GTPase-activating protein
UniProt Gene Name:GIT1  
UniProt Entry Name:GIT1_HUMAN
Anti-GIT1 Antibody [KO Validated] (CAB15437)Western blot analysis of extracts of various cell lines, using GIT1 antibody (CAB15437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-GIT1 Antibody [KO Validated] (CAB15437)Western blot analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody (CAB15437) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-GIT1 Antibody [KO Validated] (CAB15437)Immunofluorescence analysis of C6 cells using [KO] GIT1 antibody (CAB15437) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-GIT1 Antibody [KO Validated] (CAB15437)Immunofluorescence analysis of L929 cells using [KO] GIT1 antibody (CAB15437) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-GIT1 Antibody [KO Validated] (CAB15437)Immunofluorescence analysis of U-2 OS cells using [KO] GIT1 antibody (CAB15437) at dilution of 1:100. Blue: DAPI for nuclear staining.
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