Anti-GFP Antibody [H24]

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SKU:
STMA00231
Antibody Type:
Recombinant Monoclonal
Applications:
WB
Applications:
ICC/IF
Reactivity:
Species Independent
Host Species:
Mouse
Isotype:
IgG1
  • Western blot analysis with Mouse Anti-GFP Monoclonal Antibody, Clone H24 (STMA00231) showing detection of recombinant Aequorea GFP protein, his-tagged (Invitrogen A42613). Block: 2% skim milk + 2% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Jellyfish GFP Recombinant Monoclonal [H24] (STMA00231) at 1:5000 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (H&L) at 1:5000 for 30 minutes at RT.
  • Immunocytochemistry/Immunofluorescence analysis with Mouse Anti-GFP Monoclonal Antibody, Clone H24 (STMA00231). Tissue: Embryonic kidney cells (HEK-293). Species: Human. Transfection: GFP expressing plasmid, incubated for 48 hours. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 1 hour at RT.
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Description

GFP Antibody [H24]

GFP Antibody [H24] is a mouse recombinant monoclonal antibody directed against GFP, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reactivity is species independent. Supplied as IgG1, purified by Protein A.

Green fluorescent protein (GFP) is a 27 kDa reporter protein originally isolated from the jellyfish Aequorea victoria. It emits bright green fluorescence when exposed to ultraviolet or blue light, making it an indispensable tool in molecular and cellular neuroscience. GFP enables real-time visualization of gene expression, protein localization, and cellular dynamics in live cells and tissues without the need for additional substrates or cofactors. In neurodegenerative disease research, GFP is widely used to track neuronal morphology, monitor synaptic changes, and study the progression of protein aggregation in models of Alzheimer’s, Parkinson’s, and Huntington’s disease. By fusing GFP to disease-relevant proteins such as tau, α-synuclein, or huntingtin, researchers can observe pathological processes in real time and assess the efficacy of therapeutic interventions. The recombinant nature of GFP ensures high specificity, consistent expression, and minimal cytotoxicity, which enhances reproducibility across experimental systems. Additionally, its compatibility with advanced imaging techniques—including confocal and two-photon microscopy—makes GFP a cornerstone in the study of neural circuits and neurodegenerative mechanisms. As a genetically encoded fluorescent marker, GFP continues to drive innovation in neuroscience by enabling precise, dynamic, and non-invasive investigation of cellular processes underlying brain function and disease. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.

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