Epigenetics & Nuclear Signaling Antibodies 1

Anti-GEN1 Antibody (CAB10001)

(No reviews yet) Write a Review
SKU:
CAB10001
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-GEN1 Antibody
Antibody SKU:CAB10001
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 669-908 of human GEN1 (NP_872431.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:H460, MCF-7, Mouse thymus, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 669-908 of human GEN1 (NP_872431.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LQDL PLKE RIFT KLSY PQDN LQPD VNLK TLSI LSVK ESCI ANSG SDCT SHLS KDLP GIPL QNES RDSK ILKG DQLL QEDY KVNT SVPY SVSN TVVK TCNV RPPN TALD HSRK VDMQ TTRK ILMK KSVC LDRH SSDE QSAP VFGK AKYT TQRM KHSS QKHN SSHF KESG HNKL SSPK IHIK ETEQ CVRS YETA ENEE SCFP DSTK SSLS SLQC HKKE NNSG TCLD SPLP LRQR LKLR FQST
Gene ID:348654
Uniprot:Q17RS7
Cellular Location:Nucleus
Calculated MW:102kDa
Observed MW:103kDa
Synonyms:GEN1, Gen
Background:This gene encodes a member of the Rad2/xeroderma pigmentosum group G nuclease family, whose members are characterized by N-terminal and internal xeroderma pigmentosum group G nuclease domains followed by helix-hairpin-helix domains and disordered C-terminal domains. The protein encoded by this gene is involved in resolution of Holliday junctions, which are intermediate four-way structures that covalently link DNA during homologous recombination and double-strand break repair. The protein resolves Holliday junctions by creating dual incisions across the junction to produce nicked duplex products that can be ligated. In addition, this protein has been found to localize to centrosomes where it has been implicated in regulation of centrosome integrity. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:GEN1: Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.
UniProt Protein Details:

Protein type:Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 2p24.2

Cellular Component: centrosome; nucleoplasm

Molecular Function:crossover junction endodeoxyribonuclease activity; endodeoxyribonuclease activity

Biological Process: double-strand break repair via homologous recombination

UniProt Code:Q17RS7
NCBI GenInfo Identifier:290457644
NCBI Gene ID:348654
NCBI Accession:Q17RS7.2
UniProt Secondary Accession:Q17RS7,Q17RS9, Q6ZN37,
UniProt Related Accession:Q17RS7
Molecular Weight:102,884 Da
NCBI Full Name:Flap endonuclease GEN homolog 1
NCBI Synonym Full Names:GEN1, Holliday junction 5' flap endonuclease
NCBI Official Symbol:GEN1  
NCBI Official Synonym Symbols:Gen  
NCBI Protein Information:flap endonuclease GEN homolog 1
UniProt Protein Name:Flap endonuclease GEN homolog 1
Protein Family:Flap endonuclease
UniProt Gene Name:GEN1  
UniProt Entry Name:GEN_HUMAN
Anti-GEN1 Antibody (CAB10001)Western blot analysis of extracts of various cell lines, using GEN1 antibody (CAB10001) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
View AllClose

0 Reviews

View AllClose

Related Products