Epigenetics & Nuclear Signaling Antibodies 1

Anti-GEMIN8 Antibody (CAB13708)

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SKU:
CAB13708
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-GEMIN8 Antibody
Antibody SKU:CAB13708
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human GEMIN8 (NP_060326.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549, HT-29, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human GEMIN8 (NP_060326.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAV KAST SKAT RPWY SHPV YARY WQHY HQAM AWMQ SHHN AYRK AVES CFNL PWYL PSAL LPQS SYDN EAAY PQSF YDHH VAWQ DYPC SSSH FRRS GQHP RYSS RIQA STKE DQAL SKEE EMET ESDA EVEC DLSN MEIT EELR QYFA ETER HREE RRRQ QQLD AERL DSYV NADH DLYC NTRR SVEA PTER PGER RQAE MKRL YGDS AAKI QAME AAVQ LSFD KHCD RKQP KYWP VIPL KF
Gene ID:54960
Uniprot:Q9NWZ8
Cellular Location:Cytoplasm, Nucleus, gem
Calculated MW:28kDa
Observed MW:35kDa
Synonyms:GEMIN8, FAM51A1
Background:The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:GEMIN8: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
UniProt Protein Details:

Protein type:RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: cytoplasm; cytosol; nucleus; SMN complex

Molecular Function:protein binding

Biological Process: spliceosomal snRNP biogenesis

NCBI Summary:The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]
UniProt Code:Q9NWZ8
NCBI GenInfo Identifier:52782795
NCBI Gene ID:54960
NCBI Accession:Q9NWZ8.1
UniProt Secondary Accession:Q9NWZ8,Q2LJ66, Q6ZV27, C4AMC4,
UniProt Related Accession:Q9NWZ8
Molecular Weight:28,637 Da
NCBI Full Name:Gem-associated protein 8
NCBI Synonym Full Names:gem nuclear organelle associated protein 8
NCBI Official Symbol:GEMIN8  
NCBI Official Synonym Symbols:FAM51A1  
NCBI Protein Information:gem-associated protein 8
UniProt Protein Name:Gem-associated protein 8
UniProt Synonym Protein Names:Protein FAM51A1
Protein Family:Gem-associated protein
UniProt Gene Name:GEMIN8  
UniProt Entry Name:GEMI8_HUMAN
Anti-GEMIN8 Antibody (CAB13708)Western blot analysis of extracts of various cell lines, using GEMIN8 antibody (CAB13708) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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