Antibody Name:	Anti-GDI2 Antibody [KO Validated]
Antibody SKU:	CAB8615
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 296-445 of human GDI2 (NP_001485.2).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:3000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, A-549, HepG2, U-87MG, Mouse spleen, Mouse kidney, Mouse lung, Rat liver, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 296-445 of human GDI2 (NP_001485.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QVIR VICI LSHP IKNT NDAN SCQI IIPQ NQVN RKSD IYVC MISF AHNV AAQG KYIA IVST TVET KEPE KEIR PALE LLEP IEQK FVSI SDLL VPKD LGTE SQIF ISRT YDAT THFE TTCD DIKN IYKR MTGS EFDF EEMK RKKN DIYG ED
Gene ID:	2665
Uniprot:	P50395
Cellular Location:	Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:	45kDa/50kDa
Observed MW:	51kDa

Synonyms:

GDI2, HEL-S-46e, RABGDIB

Background:

GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	GDI2: Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Belongs to the Rab GDI family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein regulator, misc.; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p15 Cellular Component: cytoplasm; cytosol; focal adhesion; vesicle Molecular Function:protein binding Biological Process: regulation of small GTPase mediated signal transduction; signal transduction
NCBI Summary:	GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	P50395
NCBI GenInfo Identifier:	13638228
NCBI Gene ID:	2665
NCBI Accession:	P50395.2
UniProt Secondary Accession:	P50395,O43928, Q5SX88, Q9UQM6,
UniProt Related Accession:	P50395
Molecular Weight:	45,619 Da
NCBI Full Name:	Rab GDP dissociation inhibitor beta
NCBI Synonym Full Names:	GDP dissociation inhibitor 2
NCBI Official Symbol:	GDI2
NCBI Official Synonym Symbols:	RABGDIB; HEL-S-46e
NCBI Protein Information:	rab GDP dissociation inhibitor beta
UniProt Protein Name:	Rab GDP dissociation inhibitor beta
UniProt Synonym Protein Names:	Guanosine diphosphate dissociation inhibitor 2; GDI-2
Protein Family:	Guanosine nucleotide diphosphate dissociation inhibitor
UniProt Gene Name:	GDI2
UniProt Entry Name:	GDIB_HUMAN

	Western blot analysis of extracts of various cell lines, using GDI2 antibody (CAB8615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and GDI2 knockout (KO) 293T cells, using GDI2 antibody (CAB8615) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of NIH/3T3 cells using GDI2 antibody (CAB8615) at dilution of 1:100. Blue: DAPI for nuclear staining.