Signal Transduction Antibodies 1

Anti-GCNT3 Antibody (CAB13209)

(No reviews yet) Write a Review
SKU:
CAB13209
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-GCNT3 Antibody
Antibody SKU:CAB13209
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 27-180 of human GCNT3 (NP_004742.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T, SGC-7901, U-87MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 27-180 of human GCNT3 (NP_004742.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LKLS FRLK CDSD HLGL ESRE SQSQ YCRN ILYN FLKL PAKR SINC SGVT RGDQ EAVL QAIL NNLE VKKK REPF TDTH YLSL TRDC EHFK AERK FIQF PLSK EEVE FPIA YSMV IHEK IENF ERLL RAVY APQN IYCV HVDE KSPE TFKE AVKA II
Gene ID:9245
Uniprot:O95395
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:50kDa
Observed MW:51kDa
Synonyms:GCNT3, C2/4GnT, C24GNT, C2GNT2, C2GNTM, GNTM
Background:This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.
UniProt Protein Function:GCNT3: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. Belongs to the glycosyltransferase 14 family.
UniProt Protein Details:

Protein type:EC 2.4.1.102; EC 2.4.1.150; Glycan Metabolism - O-glycan biosynthesis; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity; acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; tissue morphogenesis; O-glycan processing; immunoglobulin production in mucosal tissue; carbohydrate metabolic process; post-translational protein modification; intestinal absorption

NCBI Summary:This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]
UniProt Code:O95395
NCBI GenInfo Identifier:74735588
NCBI Gene ID:9245
NCBI Accession:O95395.1
UniProt Related Accession:O95395
Molecular Weight:50,864 Da
NCBI Full Name:Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
NCBI Synonym Full Names:glucosaminyl (N-acetyl) transferase 3, mucin type
NCBI Official Symbol:GCNT3  
NCBI Official Synonym Symbols:GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT  
NCBI Protein Information:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; hC2GnT-M; C2GnT-mucin type; beta1,6GlcNAc-transferase; beta1,6-N-acetylglucosaminyltransferase-M; core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
UniProt Protein Name:Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
UniProt Synonym Protein Names:C2GnT-mucin type; C2GnT-M; hC2GnT-M; Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; C2/4GnT
Protein Family:Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
UniProt Gene Name:GCNT3  
UniProt Entry Name:GCNT3_HUMAN
Anti-GCNT3 Antibody (CAB13209)Western blot analysis of extracts of various cell lines, using GCNT3 antibody (CAB13209) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products