Developmental Biology

Galectin 3/LGALS3 Rabbit Monoclonal Antibody (CAB11198)

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SKU:
CAB11198
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Developmental Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin 3/LGALS3
Research Use:
Developmental Biology
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Description

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Galectin 3/LGALS3 Monoclonal Antibody (CAB11198)

The Galectin 3/LGALS3 Monoclonal Antibody (CAB11198) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with Galectin-3 in human samples and has been validated for use in Western blot applications. It specifically targets Galectin-3, allowing for precise detection and analysis in a variety of cell types, making it an excellent choice for studies in immunology, cancer research, and other fields.Galectin-3 is known for its roles in cell adhesion, proliferation, apoptosis, and inflammation, and dysregulation of its expression has been implicated in various diseases, including cancer, fibrosis, and cardiovascular disorders.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Galectin 3/LGALS3 Monoclonal Antibody
SKU:CAB11198
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0542
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MADN FSLH DALS GSGN PNPQ GWPG AWGN QPAG AGGY PGAS YPGA YPGQ APPG AYPG QAPP GAYP GAPG AYPG APAP GVYP GPPS GPGA YPSS GQPS ATGA
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:1000 - 1:6000
IHC-P1:200 - 1:800
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin 3/LGALS3
Positive Sample:HT-29, A549, MCF7, RAW 264.7, Mouse lung, Mouse spleen, Rat lung
Cellular Localization:Cytoplasm, Nucleus, Secreted.
Calculated MW:26kDa
Observed MW:28kDa

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix,the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis,innate immunity,cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID3958
RRIDAB_2861518
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Galectin 3/LGALS3 Rabbit mAb (CAB11198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon tissue using Galectin 3/LGALS3 Rabbit mAb (CAB11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Galectin 3/LGALS3 Rabbit mAb (CAB11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat colon tissue using Galectin 3/LGALS3 Rabbit mAb (CAB11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Galectin 3/LGALS3 Rabbit mAb (CAB11198) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunoprecipitation analysis of 300 μg extracts of HT-29 cells using 3 μg Galectin 3/LGALS3 antibody (CAB11198). Western blot was performed from the immunoprecipitate using Galectin 3/LGALS3 antibody (CAB11198) at a dilution of 1:1000.
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