Epigenetics & Nuclear Signaling Antibodies 1

Anti-GABPA Antibody (CAB12306)

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SKU:
CAB12306
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-GABPA Antibody
Antibody SKU:CAB12306
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABPA (NP_002031.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABPA (NP_002031.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTKR EAEE LIEI EIDG TEKA ECTE ESIV EQTY APAE CVSQ AIDI NEPI GNLK KLLE PRLQ CSLD AHEI CLQD IQLD PERS LFDQ GVKT DGTV QLSV QVIS
Gene ID:2551
Uniprot:Q06546
Cellular Location:Nucleus
Calculated MW:51kDa
Observed MW:51-56kDa
Synonyms:GABPA, E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A, RCH04A07
Background:This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:GABPA: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. Belongs to the ETS family.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: nuclear chromatin; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: cell differentiation; mitochondrion organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2010]
UniProt Code:Q06546
NCBI GenInfo Identifier:729553
NCBI Gene ID:2551
NCBI Accession:Q06546.1
UniProt Secondary Accession:Q06546,Q12939,
UniProt Related Accession:Q06546
Molecular Weight:51,295 Da
NCBI Full Name:GA-binding protein alpha chain
NCBI Synonym Full Names:GA binding protein transcription factor alpha subunit
NCBI Official Symbol:GABPA  
NCBI Official Synonym Symbols:NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07  
NCBI Protein Information:GA-binding protein alpha chain
UniProt Protein Name:GA-binding protein alpha chain
UniProt Synonym Protein Names:Nuclear respiratory factor 2 subunit alpha; Transcription factor E4TF1-60
Protein Family:GA-binding protein
UniProt Gene Name:GABPA  
UniProt Entry Name:GABPA_HUMAN
Anti-GABPA Antibody (CAB12306)Western blot analysis of extracts of various cell lines, using GABPA antibody (CAB12306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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