Immunology Antibodies 2
Anti-G3BP2 Antibody (CAB6026)
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- SKU:
- CAB6026
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-G3BP2 Antibody |
| Antibody SKU: | CAB6026 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human G3BP2 (NP_987100.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human G3BP2 (NP_987100.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DSEP ELDE ESED EVEE EQEE RQPS PEPV QENA NSGY YEAH PVTN GIEE PLEE SSHE PEPE PESE TKTE ELKP QVEE KNLE ELEE KSTT PPPA EPVS LPQE PPKP RVEA KPEV QSQP PRVR EQRP RERP GFPP RGPR PGRG D |
| Gene ID: | 9908 |
| Uniprot: | Q9UN86 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 50kDa/54kDa |
| Observed MW: | 60kDa |
| Synonyms: | G3BP2 |
| Background: |
| UniProt Protein Function: | G3BP-2: an hnRNA-binding protein and an element of the Ras signal transduction pathway. A DNA-unwinding enzyme which can unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. Binds specifically to the Ras-GTPase-activating protein (GAP120) by associating with its SH3 domain. Interacts with USP10, and may regulate it. Several alternatively spliced transcript variants have been described. |
| UniProt Protein Details: | Protein type:RNA-binding; Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 4q21.1 Cellular Component: cytoplasm Molecular Function:receptor signaling complex scaffold activity; nucleotide binding Biological Process: cytoplasmic sequestering of NF-kappaB; mRNA transport; Ras protein signal transduction |
| UniProt Code: | Q9UN86 |
| NCBI GenInfo Identifier: | 116242482 |
| NCBI Gene ID: | 9908 |
| NCBI Accession: | Q9UN86.2 |
| UniProt Secondary Accession: | Q9UN86,O60606, O75149, Q9UPA1, A8K6X1, |
| UniProt Related Accession: | Q9UN86 |
| Molecular Weight: | 482 |
| NCBI Full Name: | Ras GTPase-activating protein-binding protein 2 |
| NCBI Synonym Full Names: | GTPase activating protein (SH3 domain) binding protein 2 |
| NCBI Official Symbol: | G3BP2 |
| NCBI Protein Information: | ras GTPase-activating protein-binding protein 2; G3BP-2; GAP SH3 domain-binding protein 2; Ras-GTPase activating protein SH3 domain-binding protein 2 |
| UniProt Protein Name: | Ras GTPase-activating protein-binding protein 2 |
| UniProt Synonym Protein Names: | GAP SH3 domain-binding protein 2 |
| Protein Family: | Ras GTPase-activating protein-binding protein |
| UniProt Gene Name: | G3BP2 |
| UniProt Entry Name: | G3BP2_HUMAN |
 | Western blot analysis of extracts of HL-60 cells, using G3BP2 antibody (CAB6026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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