Immunology Antibodies 1

Anti-G3BP1 Antibody (CAB14836)

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SKU:
CAB14836
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-G3BP1 Antibody
Antibody SKU:CAB14836
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 131-330 of human G3BP1 (NP_938405.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-431, HeLa, 293T, Jurka, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 131-330 of human G3BP1 (NP_938405.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FRYQ DEVF GGFV TEPQ EESE EEVE EPEE RQQT PEVV PDDS GTFY DQAV VSND MEEH LEEP VAEP EPDP EPEP EQEP VSEI QEEK PEPV LEET APED AQKS SSPA PADI AQTV QEDL RTFS WASV TSKN LPPS GAVP VTGI PPHV VKVP ASQP RPES KPES QIPP QRPQ RDQR VREQ RINI PPQR GPRP IREA GEQG DIEP
Gene ID:10146
Uniprot:Q13283
Cellular Location:Cell membrane, Cytoplasm, Cytoplasmic granule, Nucleus, cytosol
Calculated MW:13kDa/52kDa
Observed MW:68kDa
Synonyms:G3BP1, G3BP, HDH-VIII
Background:This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
UniProt Protein Function:G3BP-1: an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Binds to the SH3 domain of Ras GTPase-activating protein (RasGAP) in proliferating cells but not in quiescent cells. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to SGs upon either arsenite or high temperature treatment. RasGAP-dependent phosphorylation of S149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. S149 phosphorylation induces partial nuclear localization. A component of a TAU mRNP complex, Interacts with USP10, and may regulate it.
UniProt Protein Details:

Protein type:RNA-binding; Helicase; Adaptor/scaffold; EC 3.6.4.12; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: focal adhesion; stress granule; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function:mRNA binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; endonuclease activity; ATP-dependent RNA helicase activity; ATP binding

Biological Process: transport; metabolic process; Ras protein signal transduction; DNA duplex unwinding

NCBI Summary:This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q13283
NCBI GenInfo Identifier:14916572
NCBI Gene ID:10146
NCBI Accession:Q13283.1
UniProt Secondary Accession:Q13283,Q5HYE9,
UniProt Related Accession:Q13283
Molecular Weight:466
NCBI Full Name:Ras GTPase-activating protein-binding protein 1
NCBI Synonym Full Names:GTPase activating protein (SH3 domain) binding protein 1
NCBI Official Symbol:G3BP1  
NCBI Official Synonym Symbols:G3BP; HDH-VIII  
NCBI Protein Information:ras GTPase-activating protein-binding protein 1; G3BP-1; GAP binding protein; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1; RasGAP-associated endoribonuclease G3BP; Ras-GTPase-activating protein SH3-domain-binding protein
UniProt Protein Name:Ras GTPase-activating protein-binding protein 1
UniProt Synonym Protein Names:ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1
Protein Family:Ras GTPase-activating protein-binding protein
UniProt Gene Name:G3BP1  
UniProt Entry Name:G3BP1_HUMAN
Anti-G3BP1 Antibody (CAB14836)Western blot analysis of extracts of various cell lines, using G3BP1 antibody (CAB14836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-G3BP1 Antibody (CAB14836)Immunohistochemistry of paraffin-embedded rat spleen using G3BP1 antibody (CAB14836) at dilution of 1:100 (40x lens).
Anti-G3BP1 Antibody (CAB14836)Immunohistochemistry of paraffin-embedded human liver cancer using G3BP1 antibody (CAB14836) at dilution of 1:100 (40x lens).
Anti-G3BP1 Antibody (CAB14836)Immunohistochemistry of paraffin-embedded mouse spleen using G3BP1 antibody (CAB14836) at dilution of 1:100 (40x lens).
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