Name: Anti-FTO Antibody [KO Validated] (CAB20992)
SKU: CAB20992
Availability: OutOfStock

Antibody Name:	Anti-FTO Antibody [KO Validated] (CAB20992)
Antibody SKU:	CAB20992
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 327-504 of human FTO (NP_001073901.1).

Application:	Western blotting, Immunohistochemistry
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, SH-SY5Y, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 327-504 of human FTO (NP_001073901.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	STGT LDYI LQRC QLAL QNVC DDVD NDDV SLKS FEPA VLKQ GEEI HNEV EFEW LRQF WFQG NRYR KCTD WWCQ PMAQ LEAL WKKM EGVT NAVL HEVK REGL PVEQ RNEI LTAI LASL TARQ NLRR EWHA RCQS RIAR TLPA DQKP ECRP YWEK DDAS MPLP FDLT DIVS ELRG QLLE AK
Cellular Location:	Nucleus, Nucleus speckle
Calculated MW:	58kDa
Observed MW:	60KDa

Synonyms:

ALKBH9, BMIQ14, GDFD

Background:

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.

	Immunohistochemistry of paraffin-embedded Human liver using FTO antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded Human esophagus using FTO antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded Human esophageal cancer using FTO antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded Mouse heart using FTO antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded Rat brain using FTO antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts from normal (control) and FTO knockout (KO) 293T cells, using FTO antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
	Western blot analysis of extracts of various cell lines, using FTO antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
	Western blot analysis of extracts of various cell lines, using FTO antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.